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Stalagmite data from Anjohibe Cave in northwest Madagascar suggest six distinct climate periods from 9.1 to 0.94 ka. Periods I and II (9.1-4.9 ka) were wetter and punctuated by a series of prominent droughts. Periods IV-VI (4-0.94 ka) were much drier and less variable. Period III (4.9-4 ka) marks the transition between wetter and drier conditions and consists of two significant droughts: the first (4.8-4.6 ka) coincides approximately with the end of the African Humid Period and the second (43-4.0 ka) may be the expression of the Northern Hemisphere 4.2 ka dry event in northwest Madagascar. Strong positive correlations between delta C-13 and delta O-18 values in Periods I-IV (r = 0.63-0.91) suggest that both isotopes were influenced by natural climate changes indicating that humans may not have been present in the area. In contrast, during Periods V (r = 0.07) and VI (r = -0.12) the "decoupling" of delta C-13 and delta O-18 might signal an impact from human activities starting around 2.5 ka. Rapid changes in climate during the early and middle Holocene, with prominent droughts lasting up to 800 years, did not kill off Madagascar's megafauna, and neither did a human population, present since the early Holocene if evidence from south Madagascar is reliable. However, many extinctions occurred under the more stable climatic conditions of the late Holocene, despite an antiphase climate relationship between northern and southcentral Madagascar. This suggests that initial human colonization, or significant increase in human population, triggered the megafaunal extinctions by hunting and destruction of megafaunal habitats. (C) 2019 Elsevier Ltd. All rights reserved.