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In order to reduce global warming potential (GWP) due to anthropogenic and natural factors in the cultivation of winterwheat and rapeseed, proved effectiveness of soil biotreatment with different composition of bio-solutions. It was reduced greenhouse gases (GHG) through life cycle impact categories from the main polluting agricultural operations on deeply lukewarm soaked soil. According to the impact diapason evaluated themain indicators such as human toxicity air (HTA), ecotoxicity water chronic (EWC), global warming potential, ecotoxicity water acute (EWA), ozone formation (OF), human toxicity water (HTW), ecotoxicity soil chronic (ESC), human toxicity soil (HTS), terrestrial eutrophication (TE), acidification (A). Assessed researches for three seasons which carried out in production experimental areas. It was appreciated in interrelated stages according to LST EN ISO 14040: 2007 standard. Mass balance for one functional unit (FU) was tonne of wheat and rapeseed. SimaPro 8.05 life cycle assessment (LCA) Software was used for comparing soil biotreatment efectiveness using different bio-solutions and its mixes with control. The aim of the assessment - to prove the soil biotreatment effectiveness for reducing main life cycle indicators from tillage operations. It was identificated that phase of field operation is one of the main factor to the global impact. Disc harrowing consists approximately 26%, ploughing - 40% of all operations. Identified effectiveness of soil biotreatment, wheat and rapeseed rotation for reduction of global warming potential. Discovered reduction interdependencies of main life cycle assessement impact categories. The largest CO2 eq reducing established from primary - disc harrowing 12-15 cm and secondary - ploughing 23-25 cm soil tillage. It was fixed till approximately 15% in mixed bacterial and non-bacterial bio-solution after first soil biotreatment. Till approximately 8% CO2 eq reduction was in mixed bio-solutions after second biotreatment. The percentage highest soil biotreatment effectiveness till approximately 30% assessed after third biotreatment compared to usual technology. Soil biotreatment effectiveness in reducing green house gases (GHG) proved first year in 86%, second year in 43%, and third year in 71% of all used bio-solutions. (C) 2019 Elsevier B.V. All rights reserved.