Unreasonable use of biological resources by human beings and climate change have the negative impacts on biodiversity at global scale,thus the hotspot of biodiversity zone has been the major focus in researches.Based on the condition of biodiversity in Guizhou province,this paper collected the index of habitat quality,biodiversity and landscape diversity as the evaluation factors to calculate the index of biodiversity,and further to locate the hotspots of biodiversity in Guizhou province via the method of spatial correlation.The area of biodiversity hot zone in Guizhou reached 37 662.68 km~2,accounting for 21.37% of the study area,which covered 582 indicator species,accounting for 94% of all indicator species.There are six zones of biodiversity hotspot in Guizhou province,such as Northeast of Guizhou-Fanjingshan,the center of Fuodingshan in Gaozhongshan and Zhongshan;Daloushan,Chishui river,valley of Xishui river in North Guizhou;Leigongshan,Yueliangshan and valley of Liujiang in Southeast of Guizhou;Libo-Dushan karst mountainous area;the plateau lakes of Weiningcaohai;south Panjiang and north Panjiang and valley of Hongshui river in southwest of Guizhou.According to the analysis,we suggest it is necessary that the navigation and dynamic monitoring of biodiversity should been carried out in Guizhou,and a database of protection zone should be established by 3Smethod with respect to the better protection of biodiversity and ecological restoration.