Using the daily meteorological data observed by 50 stations in Qinghai Province from 1961 to 2010,are employed to analyze the spatial-temporal characteristics of Comfort of Human Body Index (I_(CHB)) and weights of factors. The results indicated that: (1) Main comfort degree in Qinghai are frigid, cold, cool, pleasantly cool and comfortable, it's overall character is cold and cool. There are large differences of average number of annul days in each region and degree. (2) January is the most uncomfortable month, followed by December while July is the most comfortable month, followed by August and June. (3) I_(CHB) increased significantly in Qinghai Province from 1961 to 2010, consistent with the climates change on the Qinghai-Tibet Plateau. I_(CHB) of each region increased significantly, which Qaidam region increased the most significantly, the trend in Eastern agricultural region is the same as in Qaidam region in spring and autumn, Qinghai Lake region is similar to Qingnan Pastoral. Upward trend of autumn is higher than that of spring in each area. Inter-annual fluctuation range of I_(CHB) is large in each season in Qingnan Pastoral. I_(CHB) in Qinghai Lake region is similar to Qingnan Pastoral in winter. (4) Seasonal differences of I_(CHB) are significant. Summer is the highest and winter is the lowest, spring is slightly higher than autumn. I_(CHB) of each season in recent 50 a increased significantly. The fluctuation range is larger in winter. (5) Temperature is positively correlated with I_(CHB) while humidity and wind speed is negative with it. Temperature is the main factor affect the I_(CHB), wind speed and humidity effect the I_(CHB) by temperature, and influence of wind speed is larger than humidity. The greater negative impact factors in Eastern agricultural region and Qaidam region is wind speed, while in Qinghai-Lake region and Qingnan Pastoral is humidity.