With the Ebinur Lake as the research area, over sixty years data of hydrology and meteorology were taken to analyze the influence of the climate change and human activities on the water resources in the Ebinur Lake Basin. The results show that (1) there is a rise trend of the runoff in the three main streams of the Ebinur Lake in the recent 60 years; (2) due to the impact from the human activities, both the lake area and water appear a decreasing trend in the recent 60 years; (3) the negative effect of the human activity influence is more than the positive effect of the climate change, and the human activity increasing has let to the water surface decreasing of the Ebinur Lake.