globalchange  > 气候变化与战略
CSCD记录号: CSCD:5678592
论文题名:
胁迫下的细胞程序性死亡鉴定有助于植物抗逆性
其他题名: Identifying stress-related programmed cell death improves research on plant resistance
作者: 徐凌翔; 陈壬杰; 许海锦; 周元昌; 吴为人
刊名: 科学通报
ISSN: 0023-074X
出版年: 2016
卷: 61, 期:8, 页码:22-28
语种: 中文
中文关键词: 坏死 ; 类半胱天冬酶 ; DNA片段化 ; 类病斑突变体 ; 自噬 ; 活性氧
英文关键词: necrosis ; metacaspase ; DNA laddering ; lesion mimic mutant ; autophagy ; reactive oxygen species
WOS学科分类: PLANT SCIENCES
WOS研究方向: Plant Sciences
中文摘要: 细胞程序性死亡(PCD)在植物生存和进化上有重要的意义. 近几年, 遗传学、生理学、分子与细胞生物学技术的发展以及动物细胞理论的平行性对植物PCD的鉴定起了很大推动作用, 但胁迫相关的PCD的鉴定仍有较大的复杂性. 当今植物PCD的鉴定主要以探针染色分析核形为主, 多种方法结合, 但还需要更加可靠的表征和表征的量化. 逆境下PCD的鉴定主要在3个方面有潜在应用价值: (ⅰ)多重抗性间的关联研究; (ⅱ)抗性遗传资源使用; (ⅲ)种质资源保存. 本文主要结合实践中的PCD鉴定的应用前景来综述当前胁迫条件下植物PCD研究的进展.
英文摘要: Programmed cell death (PCD) is essential to the survival and evolution of plants. Recently, the advance in the technology of plant genetics, physiology, molecular and cytological biology supports the study on the phenomena and mechnisms of plant PCD. In respect of phenomena, the significant progress includes the classification of two distinguish types of plant PCD: vacuolar PCD and necrotic PCD. The outstanding progress in mechnisms includes the discovery of the structure of a proteinase family: metacaspases, along with their role in survival/death decision or cell-death type controlling. However, complications in recognizing plant PCD still persist for little is genetically conserved between plants and animals PCD-regulation, so recognition of plant PCD depends on its parallels with more adequately studied animal PCD. Stress-related PCD is even more complicated comparing with developmental PCD because it contains briefly necrotic PCD and features of vacuolar PCD, while developmental PCD can be exclusively attributed to vacuolar PCD. Moreover, stress-related PCD is likely to be confused with non-programmed cell death. Identification of plant PCD consists of multiple methods including molecular biological methods, morphological methods and physiological methods. Up to date, fluorescent probe staining nucleis, which is a combination of molecular and morphological method becomes the most popular technique to label plant PCD and its effect is reinforced by other probes which label cell death or autophagic activity, morphological observation with electron microscope and DNA agarose gel electrophoresis. Still, both more reliable markers and quantification of marked events is required. Stress-related PCD is becoming more important against both biotic and abiotic stress and its identification would potentially improve the research in three respects: (i) correlating the resistances to multiple stresses; (ii) the utilization of disease-resistant genetic resources and (iii) the preservation of germplasm. These applications assist each other and would reciprocally improve the comprehension of the patterns and mechanisms in plant PCD. Stree-related PCD in plant is most profoundly studied in the hypersensitive response (HR) during effectortriggered-immunity (ETI) with recognition of pathogens. However, PCD is by far not confined only within ETI but also associated with a great variety of resistance. Loci related to PCD or other PCD-related process like salicylic or jasmonic metabolism, reactive oxygen species (ROS) scavenging, redox homeostasis and calcium signaling often contribute to resistance to a great variety of factors. Therefore PCD identification has the potentiality to help in selection in crop breeding and to decipher regulatory pathways and network for stress resistance. One popular application of PCD in crop breeding is the lesion mimic mutants (LMMs). LMMs exhibit intrinsic PCD and can be disease resistant usually at the cost of production. The identification of phenotype concerning PCD could help selecting most fitable LMM genotype to balance production and resistance. LMMs in turn help deciphering PCD pathways. Spatial and temporal heterogeneity exists in LMMs and such heterogeneity is comparatively well studied in PCD in germplasm preservation including the storage of seeds, tissues or cells. Decline in vigor of germplasm corelates with the proliferating of PCD cells, which probably include a process of survival/death decision. Therefore timing, counting and locating PCD cells would assist in monitoring germlasm vigor, understanding the process of deteriotation and deciding whether and when to rescue germplasm resources. As a result of global climate change and population growing pressure and various secondary problems derived from these, breeding stress-resistant crops and preserving germplasm become urgent and emphasize the indentification of stress-related PCD.
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/151049
Appears in Collections:气候变化与战略

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作者单位: 福建农林大学, 作物遗传育种与综合利用教育部重点实验室, 福州, 福建 350002, 中国

Recommended Citation:
徐凌翔,陈壬杰,许海锦,等. 胁迫下的细胞程序性死亡鉴定有助于植物抗逆性[J]. 科学通报,2016-01-01,61(8):22-28
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