Climate change is a process that is driven by multiple factor changes. The response of soil CH_4 flux on climate change is an integrated effect of multiple factors. Atmospheric temperature and nitrogen deposition are constantly increasing in Inner Mongolia at recent years, therefore, it is necessary to study the effects of global warming and nitrogen deposition on soil CH_4 flux in grassland ecosystem. To study the effects of warming, nitrogen deposition and their interaction on soil CH_4 flux in Stipa breviflora desert steppe, a static chamber method was used to measure soil CH_4 flux during two growing season (from May to October) in 2013 and 2014 under the long-term (since 2006) warming and nitrogen addition. Meanwhile, soil temperature and soil moisture were continuously measured at the depth of 10 cm. The results showed that soil temperature and soil moisture were significantly increased in the warming plots, but soil moisture in the nitrogen addition plots was significantly decreased (P < 0.0001). Mean soil CH_4 uptake during the growing season was 40.2~50.5 mug?m~(-2)?h~(-1) in Stipa breviflora desert steppe. The soil CH_4 uptake was significantly influenced by year (P =0.0097). The warming, nitrogen addition and their interaction did not significantly influence the soil CH_4 uptake (P >0.05). There are different relationships between soil CH_4 uptake and soil temperature in the different years. In 2013, a significant linear relationship was found between soil CH_4 uptake and soil temperature (P=0.0291), but there was a significant quadratic polynomial relationship in 2014 (P=0.0396). The influence of soil moisture on soil CH_4 uptake was described by a quadratic polynomial relationship in 2013 (P=0.0124), but there was no relationship between them in 2014. The integrated influence of soil temperature and soil moisture or monthly precipitation on soil CH_4 uptake (R~2: 0.37~0.76) was higher than any single factor (R~2: 0.20~0.34). The results indicated that Stipa breviflora desert steppe was an atmospheric CH_4 sink during the growing season. Atmospheric warming and nitrogen deposition had no influence on soil CH_4 uptake in Stipa breviflora desert steppe.