Conservation and sustainable use of marine and coastal biodiversity is an important part in the Conservation on Biological Diversity (CBD). By reviewing the course of negotiation, this paper finds that focal issues include: (1) Actions to address the impacts caused by human activities and global change on marine and costal biodiversity; (2) Tools for the conservation and sustainable use of marine and coastal biodiversity resources; and (3) Marine protected areas and Ecologically or Biologically Significant Marine Areas (EBSAs). These negotiations may have impacts on marine biodiversity conservation, and the process of establishing a network of global marine protected areas and international marine biodiversity conservation, as well as socioeconomic development in maritime countries. In the future, China should accelerate research on marine related science and technology, become actively involved in the course of negotiations, and publicize achievements in the implementation of the Convention.