In order to effectively evaluate the influence of human activities on the resource and environment, this paper constructed an resource and environment pressure evaluation system based on footprint family,and calculated the ecological footprint,carbon footprint,water footprint,and resource and environment pressure in Qinghai Province. The results showed that from 1990 to 2013, the ecological footprint per capita in Qinghai Province increased from 1.32 hm~2 to 3.32 hm~2,and biological capacity per capita decreased from 2.33 hm~2 to 2.07 hm~2. Since 2006 there was ecological deficit. However,the biomass surplus existed during 1990-2013. The carbon footprint per capita increased from 5.82 t to 15.85 t,which had been more than 7.93 times of the target to address climate change. The water footprint per capita decreased from 967.67 m~3 to 732.05 m~3. Since 1990,the ecological pressure was at low grade (Ⅰ_b),greenhouse gas (GHG) emissions pressure was enhanced from low grade (Ⅰ_b) to above-average grade (Ⅱ_b),the water pressure was at very low grade (Ⅰ_a),the resource and environment pressure increased from very low grade (Ⅰ_a) to low grade (Ⅰ_b). The rate of contribution of ecological pressure and water resource pressure to resources and environment pressure gradually decreased,whereas,the rate of contribution of GHG emission pressure to resources and environment pressure gradually increased. The resource and environment pressure had spatial heterogeneity. In 2013,it was at very high grade (Ⅲ_b) in Xining City,at above-average grade (Ⅱ_b) in Haidong City,at below-average grade (Ⅱ_a) in Haixi State and at very low grade (Ⅰ_a) in the rest regions. The main resource and environment pressure source also had obvious spatial heterogeneity. In future,differentiation strategies should be taken in the decompression progress.