Land use change across Northeast China experienced a significant spatiotemporal variation between 1990 and 2010. Northeast China is one of the most sensitive regions to global climate change. A better understanding of the effects of land use change and climate change on water yield is of great importance to water resource planning and sustainable management. Based on the land use data in 1990,2000,and 2010,meteorological data,and soil data,InVEST model was used to analyze the spatiotemporal changes in water yield in Northeast China from 1990 to 2010. Structural equations were used to explore the influence of climate change and land use change on water yield in Northeast China. The results showed that the water yield in Northeast China had a trend of increase after an initial decrease in the 20 years. In addition,montane regions of Eastern Liaoning Province and Changbai Mountain area had the highest water yield,while the plain area had the least water yield. The precipitation was estimated to be the main factor leading to the spatiotemporal variation of water yield in Northeast China,while temperature and land use change had a minor influence on water yield.