By the analysis of 44 soil samples at the borehole ZK001 (44。48'44"N,114°44'44"E,the depth of 260.00 m)in the Manite depression, Erlian basin, four palynological assemblages are recognized from the bottom to the top. These four assemblages are named as Pinuspollenites - Cicatricosisporites - Lycopodiaceae - Pilosisporites - Triletes (depth of 260.00 m to 235.21 m), Pinuspollenites - Piceaepollenites - Chenopodipollis - Artemisiaepollenites -Cicatricosisporites - Triletes (depth of 235.21 m to 176.00 m), Pinuspollenites - Chenopodipollis -Artemisiaepollenites-Triletes - Lycopodiaceae (depth of 176.00 m to 84.56 m),Pinuspollenites - Chenopodipollis -Artemisiaepollenites -Triletes (depth of 84.56 m to 15.60 m),respectively. According to the results of sporopollen analysis and sedimentary lithology, vegetation succession and paleoclimate evolution in the studying area has been divided into two stages. At the depth of 260.00 m to 84.56 m,the characteristics of palynological assemblage represent the coniferous and broad-leaved mixed forest-grassland vegetation type,which reflects the climate changes from hot-humid, warm-humid to warm-arid. At the depth of 84.56 m to 15.60 m, the features of palynological assemblage represent the vegetation of sparse forest grassland type, which reflects cold-arid climate. The stratigraphic age in borehole of the research area has been discussed by comparing the characteristic of the palynological assemblages and formation lithology in adjacent areas. The results show that the layer can be defined as Early Cretaceous Damoguaihe Formation (K_1d) between 260.00 m and 84.56 m and Paleogene Irdin Manha Formation(Ey) between 84.56 m and 15.60 m. This study has great significance for the division of Paleogene and Cretaceous, which provides a new demonstration for the paleoclimate changes.