globalchange  > 气候变化事实与影响
CSCD记录号: CSCD:5579582
论文题名:
低温对大鼠全脑缺血再灌注时海马内质网应激的影响
其他题名: Effect of hypothermia on hippocampal endoplasmic reticulum stress during global cerebral ischemia-reperfusion in rats
作者: 高宇; 金利; 吴淋; 蔡慎权; 范婧婧; 仲浩; 段满林; 徐建国
刊名: 中华麻醉学杂志
ISSN: 0254-1416
出版年: 2015
卷: 35, 期:9, 页码:1146-1149
语种: 中文
中文关键词: 低温,人工 ; 内质网 ; 应激 ; 脑 ; 再灌注损伤
英文关键词: Hypothermia,induced ; Endoplasmic reticulum ; Stress ; Brain ; Reperfusion injury
WOS学科分类: MEDICINE GENERAL INTERNAL
WOS研究方向: General & Internal Medicine
中文摘要: 目的评价低温对大鼠全脑缺血再灌注时海马内质网应激的影响。方法清洁级健康雄性SD大鼠54只,体重280~320 g,采用随机数字表法分为3组(n=18):假手术组(S组)、全脑缺血再灌注组(I/R组)和低温组(H组)。采用经食管心脏起搏诱发心跳骤停+心肺复苏制备大鼠全脑缺血再灌注损伤模型。H组再灌注即刻体表降温,10 min之内将体温降至32~34 ℃,维持4 h,其余组体温维持36~37 ℃。于再灌注24 h时行神经功能缺陷评分,随后处死大鼠取双侧海马,采用Western blot法检测葡萄糖调节蛋白78(GRP78)和caspase-12的表达。取全脑组织行HE染色,观察海马CAI区锥体细胞形态结构,免疫组化染色法计数GRP78和caspase-12的阳性细胞。结果与S组比较,I/R组和H组再灌注24 h时神经功能缺陷评分升高,海马CA1区正常锥体细胞数减少,GRP78和caspase-12表达上调,阳性细胞数增多(P<0.05);与I/R组比较,H组再灌注24 h时神经功能缺陷评分降低,海马CA1区正常锥体细胞数增多,GRP78表达上调,阳性细胞数增多,caspase-12表达下调,阳性细胞数减少(P<0.05)。结论低温可通过抑制海马内质网应激减轻大鼠全脑缺血再灌注损伤。
英文摘要: Objective To evaluate the.effect of hypothermia on hippocampal endoplasmic reticulum stress during global cerebral ischemia-reperfusion (I/R) in rats. Methods Fifty-four healthy male Sprague-Dawley rats, weighing 280-320 g, were randomly divided into 3 groups (n = 18 each) using a random number table: sham operation group (group S), global cerebral I/R group (group I/R) and hypothermia group (group H). Cardiac arrest was induced with transoesophageal cardiac pacing followed by cardiopulmonary resuscitation to establish the global cerebral I/R model in anesthetized rats in I/R and H groups. In group H, the body temperature was cooled down to 32-34 ℃ within 10 min starting from the beginning of reperfusion, and maintained at this level for 4 h, and the body temperature was maintained at 36-37 ℃ in the other groups. Neurological deficit was scored at 24 h of reperfusion, and the rats were then sacrificed, and bilateral hippocampi were immediately removed for determination of glucose-regulated protein 78 (GRP78) and caspase-12 expression (by Western blot). The brains were removed and cut into sections which were stained with haematoxylin and eosin to examine the morphological structures of pyramidal cells in hippocampal CA1 region (under microscope). Immunohistochemical staining was used to count GRP78 and caspase-12 positive cells. Results Compared with group S, the neurological deficit score was significantly increased at 24 h of reperfusion,the number of normal pyramidal cells in hippocampal CA1 region was decreased, the expression of GRP78 and caspase-12 was up-regulated,and the number of GRP78 and caspase-12 positive cells was increased in I/R and H groups (P<0.05). Compared with group I/R,the neurological deficit score was significantly decreased at 24 h of reperfusion,the number of normal pyramidal cells in hippocampal CA1 region was increased, the expression of GRP78 and caspase-12 was down-regulated,and the number of GRP78 and caspase-12 positive cells was decreased in group H (P<0.05). Conclusion Hypothermia can attenuate global cerebral I/R injury through inhibiting hippocampal endoplasmic reticulum stress in rats.
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/156745
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作者单位: 南京大学医学院附属金陵医院麻醉科, 210000

Recommended Citation:
高宇,金利,吴淋,等. 低温对大鼠全脑缺血再灌注时海马内质网应激的影响[J]. 中华麻醉学杂志,2015-01-01,35(9):1146-1149
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