DOI: 10.1016/j.scitotenv.2020.137639
论文题名: Subchronic oral mercury caused intestinal injury and changed gut microbiota in mice
作者: Zhao Y. ; Zhou C. ; Wu C. ; Guo X. ; Hu G. ; Wu Q. ; Xu Z. ; Li G. ; Cao H. ; Li L. ; Latigo V. ; Liu P. ; Cheng S. ; Liu P.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 721 语种: 英语
英文关键词: Gut microbiota
; Injury
; Mercury
; Mice
Scopus关键词: Bacteriology
; Cell death
; Chlorine compounds
; Gene expression
; High resolution transmission electron microscopy
; Mammals
; Mercury compounds
; Metabolism
; Potable water
; Correlation analysis
; Growth and development
; Gut microbiota
; High-throughput sequencing
; Histopathological examinations
; Injury
; Mice
; Microbial populations
; Mercury (metal)
; Coprococcus
; Helicobacter
; Mus
; Oscillospira
; Salinicoccus
英文摘要: Mercury is a key global pollutant, yet the mechanism by which mercury-exposure causes intestinal injury is not clear, we aimed to investigate the mechanism of intestinal injury and gut microbiota changes caused by mercury-exposure. Twelve Kunming mice were divided into two groups (n = 6), and the two groups were treated with 0 mg/L and 80 mg/L HgCl2 in drinking water for 90 days respectively. Our results showed that mercury-exposure prominently effected body weight gain and glucose levels. The mercury-exposed mice showed intestinal injury, which was diagnosed by Histopathological Examination and Transmission Electron Microscopy. Meanwhile, RT-PCR indicated that mercury-exposure significantly increased the expression of pro-apoptotic genes including Bax, JNK, ASK1, caspase3 and TNF-α, and significantly decreased the expression of the anti-apoptotic gene Bcl-2. Furthermore, high-throughput sequencing analysis showed that at the genus level some microbial populations including Coprococcus, Oscillospira and Helicobacter were significantly increased whereas some microbial populations including Lgnatzschineria, Salinicoccus and Bacillus were significantly decreased. Moreover, PICRUSt analysis revealed potential metabolic changes. Correlation analysis indicated that microorganisms were significantly correlated with apoptotic gene expression. In summary, our results indicated that mercury-exposure affected the growth and development of mice, induced intestinal microbiota dysbiosis and metabolic disorder, and aggravated apoptosis in mice. © 2020 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158089
Appears in Collections: 气候变化与战略
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作者单位: Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China; Department of Mathematics and Statistics, Wright State University, Dayton, OH 45435, United States; Ganzhou Vocational and Technical College, Ganzhou City, Jiangxi Province, China; Jiangxi Biological Vocational College, Nangchang City, Jiangxi Province, China
Recommended Citation:
Zhao Y.,Zhou C.,Wu C.,et al. Subchronic oral mercury caused intestinal injury and changed gut microbiota in mice[J]. Science of the Total Environment,2020-01-01,721