DOI: 10.1016/j.scitotenv.2020.137733
论文题名: Evaluating bivalve cytoprotective responses and their regulatory pathways in a climate change scenario
作者: Franzellitti S. ; Prada F. ; Viarengo A. ; Fabbri E.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 720 语种: 英语
英文关键词: Antibiotic
; Marine mussel
; Metal
; Stress response
; Temperature
; Transcriptional control
Scopus关键词: Antibiotics
; Copper compounds
; Ecosystems
; Gene expression
; Glycoproteins
; Inorganic chemicals
; Metals
; Modulation
; Molluscs
; Organic chemicals
; Physiology
; Pollution
; Seawater effects
; Temperature
; Climate change scenarios
; Marine mussels
; Mytilus galloprovincialis
; Oxytetracyclines (OTC)
; Physiological temperature
; Stress response
; Transcriptional control
; Transcriptional modulation
; Climate change
; acclimation
; bivalve
; climate change
; climate effect
; copper
; environmental disturbance
; physiological response
; temperature effect
; Animalia
; Bivalvia
; Mytilidae
; Mytilus galloprovincialis
英文摘要: Temperature is a relevant abiotic factor affecting physiological performance and distribution of marine animals in natural environments. The changes in global seawater temperatures make it necessary to understand how molecular mechanisms operate under the cumulative effects of global climate change and chemical pollution to promote/hamper environmental acclimatization. Marine mussels are excellent model organisms to infer the impacts of those anthropogenic threats on coastal ecosystems. In this study, Mediterranean mussels (Mytilus galloprovincialis) were exposed to different concentrations of the metal copper (Cu as CuCl2: 2.5, 5, 10, 20, 40 μg/L) or the antibiotic oxytetracycline (OTC: 0.1, 1, 10, 100, 1000 μg/L) at increasing seawater temperatures (16 °C, 20 °C, 24 °C). Transcriptional modulation of a 70-kDa heat shock protein (HSP70) and of the ABC transporter P-glycoprotein (P-gp, encoded by the ABCB gene) was assessed along with the cAMP/PKA signaling pathway regulating both gene expressions. At the physiological temperature of mussels (16 °C), Cu and OTC induced bimodal changes of cAMP levels and PKA activities in gills of exposed animals. A correlation between OTC- or Cu- induced changes of PKA activity and expression of hsp70 and ABCB was observed. Temperature increases (up to 24 °C) altered ABCB and hsp70 responses to the pollutants and disrupted their relationship with cAMP/PKA modulation, leading to loss of correlation between the biological endpoints. On the whole, the results indicate that temperature may impair the effects of inorganic and organic chemicals on the cAMP/PKA signaling pathway of mussels, in turn altering key molecular mediators of physiological plasticity and cytoprotection. © 2020 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158092
Appears in Collections: 气候变化与战略
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作者单位: Animal and Environmental Physiology Laboratory, Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Ravenna, Italy; Fano Marine Center, Department of Biological, Geological, and Environmental Sciences (BiGeA), University of Bologna, Fano, Italy; Marine Science Group, Department of Biological, Geological, and Environmental Sciences (BiGeA), University of Bologna, Bologna, Italy; Ecotoxicology and Environmental Safety Unit, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy
Recommended Citation:
Franzellitti S.,Prada F.,Viarengo A.,et al. Evaluating bivalve cytoprotective responses and their regulatory pathways in a climate change scenario[J]. Science of the Total Environment,2020-01-01,720