globalchange  > 气候变化与战略
DOI: 10.1016/j.postharvbio.2020.111137
Title:
iTRAQ-based quantitative proteomic analysis of peach fruit (Prunus persica L.) at different ripening and postharvest storage stages
Author: Jiang L.; Kang R.; Feng L.; Yu Z.; Luo H.
Source Publication: Postharvest Biology and Technology
ISSN: 9255214
Publishing Year: 2020
Volume: 164
Language: 英语
Keyword: iTRAQ ; Peach fruit ; Protein quantification ; Proteome ; Ripening
English Abstract: To better understand the global and dynamic changes in peach (Prunus persica L. cv. Xiahui 8) fruit protein expression, an efficient and reliable proteomic technique, iTRAQ, was used to investigate peach fruit proteome from day -7 to day 8 during storage period at 25 ± 1 °C. A total of 387 proteins with more than 1.2-fold abundance changes were observed. These differentially expressed proteins (DEPs) are mainly related to 17 functional categories. DEPs in day -7 were mainly responsible for sugar and carbohydrate syntheses, protein translation, expression and modification, and maintenance of osmotic balance in high ion and material transport capacities and strong redox reaction environment. Harvesting induced the increase of (small) heat shock protein. Upon harvest, the increased level of respiration rates on day 4 reached the highest level. polygalacturonase (PG), pectase lyase (PL), pectinesterase (PE), chorismate mutase (CM), peptidyl-prolyl isomerase (PPIase) and phytoene synthase (PSY) showed increased abundances and reached a peak at day 6, which significantly positive correlation with 9-cis-epoxycarotenoid dioxygenase (NCED) protein, accelerated fruit softening and metabolism of protein, amino acid and lipid. The emergence of eukaryotic translation initiation factor 5A (EIF5A) on day 8 signified the intensification of apoptosis. Our study facilitated a comprehensive understanding of the complex proteomic reprograming and ripening-associated mechanisms that could be used for endeavors of breeding and postharvest processing to improve peach fruit quality. © 2020
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Document Type: 期刊论文
Identifier: http://119.78.100.158/handle/2HF3EXSE/158155
Appears in Collections:气候变化与战略

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Affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, China

Recommended Citation:
Jiang L.,Kang R.,Feng L.,et al. iTRAQ-based quantitative proteomic analysis of peach fruit (Prunus persica L.) at different ripening and postharvest storage stages[J]. Postharvest Biology and Technology,2020-01-01,164
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