DOI: 10.1016/j.scitotenv.2020.137359
论文题名: Metabolomic, functional, and ecologic responses of the common freshwater fungus Neonectria lugdunensis to mine drainage stress
作者: Seena S. ; Sobral O. ; Cano A.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 718 语种: 英语
英文关键词: Cellobiohydrolase I (CbhI) gene
; Gene expression
; Litter decomposition
; Metabolomics
; Metals
; Stress
Scopus关键词: Ascorbic acid
; Contamination
; Drainage
; Fungi
; Gene expression
; High performance liquid chromatography
; Lipids
; Mass spectrometry
; Metals
; Stresses
; Cellobiohydrolase I
; Contaminated streams
; Ecological performance
; Functional signatures
; Litter decomposition
; Metabolomic profiling
; Metabolomics
; Ultra high performance liquid chromatography-mass spectrometries
; River pollution
; amino acid
; cellulose 1,4 beta cellobiosidase
; lipid
; lysophosphatidylcholine
; metal
; protein CbhI
; triacylglycerol
; unclassified drug
; decomposition
; functional response
; fungus
; gene expression
; litter
; metabolism
; metal
; mine drainage
; streamwater
; toxicity
; acid mine drainage
; aquatic species
; Article
; CbhI gene
; concentration (parameter)
; down regulation
; ecotoxicity
; environmental exposure
; fatty acid desaturation
; freshwater species
; fungal gene
; fungus hyphae
; fungus isolation
; gene expression profiling
; gene function
; heavy metal poisoning
; Hyphomycetes
; litter decomposition
; mass spectrometry
; metabolic fingerprinting
; Neonectria lugdunensis
; nonhuman
; Portugal
; priority journal
; protein function
; stream (river)
; ultra performance liquid chromatography
; water pollution
; water supply
; Portugal
; Fungi
; Neonectria
英文摘要: Metal contamination of watersheds is a global problem. Here, we conducted litter decomposition studies with Neonectria lugdunensis, a cosmopolitan aquatic fungus. Fungal isolates from four reference (non-impacted) and six metal-contaminated streams (due to mine drainage) were exposed to mine drainage and reference stream waters in Central Portugal. Impact of mine drainage waters on N. lugdunensis hyphae was investigated by performing metabolomic profiling of 200 lipids and 25 amino acids (AA) with ultra-high performance liquid chromatography-mass spectrometry. In parallel, functional response of N. lugdunensis isolates was assessed through expression profiles of a functional gene, cellobiohydrolase I (CbhI). Ecological performance via leaf mass loss was also determined. Exposure to mine drainage waters altered the concentration of numerous AA and lipids. Most strikingly, a gradual increase in the concentration of the triacylglycerols (TAG) with shorter acyl chains and lesser unsaturation was observed after the exposure to mine drainage waters. In addition, the changes in the concentration of numerous TAG, lysophosphatidylcholines, and AA were more significant in the isolates from the metal-contaminated streams after exposure to mine drainage water. CbhI gene of the isolates from reference streams was down-regulated by metal stress, while those from metal-contaminated streams remained unaffected. Finally, leaf mass loss was influenced by both exposure to mine drainage waters and the origin of isolates. Overall, our study demonstrates unique functional signatures displayed by fungi under metal stress and the relevant role that fungal AA and lipids play to cope with metal toxicity. © 2020
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158193
Appears in Collections: 气候变化与战略
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作者单位: MARE - Marine and Environmental Sciences Centre, University of Coimbra, Coimbra, PT-3004-517, Portugal; CBMA - Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal; ONE WAY LIVER, S.L., Parque Tecnológico de Bizkaia, edif.502- plta 0, Derio, Bizkaia 48160, Spain
Recommended Citation:
Seena S.,Sobral O.,Cano A.. Metabolomic, functional, and ecologic responses of the common freshwater fungus Neonectria lugdunensis to mine drainage stress[J]. Science of the Total Environment,2020-01-01,718