globalchange  > 气候变化与战略
DOI: 10.1016/j.envint.2020.105600
Arsenic concentrations, diversity and co-occurrence patterns of bacterial and fungal communities in the feces of mice under sub-chronic arsenic exposure through food
Author: Wang J.; Hu W.; Yang H.; Chen F.; Shu Y.; Zhang G.; Liu J.; Liu Y.; Li H.; Guo L.
Source Publication: Environment International
ISSN: 1604120
Publishing Year: 2020
Volume: 138
Language: 英语
Keyword: Arsenic ; Food exposure ; Gut microbiome ; Rice
Scopus Keyword: Arsenic ; Bacteria ; Detoxification ; Fungi ; Gene encoding ; Inductively coupled plasma mass spectrometry ; Mammals ; Mass spectrometers ; Metabolism ; Potable water ; RNA ; Alpha and beta diversities ; Arsenic concentration ; Arsenic poisoning ; Co-occurrence pattern ; Metabolic pathways ; Microbiological analysis ; Microbiome ; Rice ; Chemical contamination ; arsenic ; concentration (composition) ; DNA ; food intake ; microbial community ; pollution exposure ; rice ; species diversity ; Amanitaceae ; Animalia ; Bacteria (microorganisms) ; Candida ; Deltaproteobacteria ; Fungi ; Fusarium ; Mus ; Polynucleobacter ; Saccharomyces
English Abstract: Background: Arsenic, a global pollutant and a threshold-free primary carcinogen, can accumulate in rice. Previous studies have focused on arsenic poisoning in drinking water and the effects on gut microbes. The research on arseniasis through food, which involves the bio-transformation of arsenic, and the related changes in gut microbiome is insufficient. Method: Mice were exposed from animal feed prepared with four arsenic species (iAsIII, iAsV, MMA, and DMA) at a dose of 30 mg/kg according to the arsenic species proportion in rice for 30 days and 60 days. The levels of total arsenic (tAs) and arsenic species in mice feces and urine samples were determined using ICP-MS and HPLC-ICP-MS, respectively. 16S rRNA and ITS gene sequencing were conducted on microbial DNA extracted from the feces samples. Results: At 30 days and 60 days exposure, the tAs levels excreted from urine were 0.0092 and 0.0093 mg/day, and tAs levels in feces were 0.0441 and 0.0409 mg/day, respectively. We found significant differences in arsenic species distribution in urine and feces (p < 0.05). In urine, the predominant arsenic species were iAsIII (23% and 14%, respectively), DMA (55% and 70%, respectively), and uAs (unknown arsenic, 14% and 10%, respectively). In feces, the proportion of major arsenic species (iAsV, 26% and 21%; iAsIII, 16% and 15%; MMA, 14% and 14%; DMA, 19% and 19%; and uAs, 22% and 29%, respectively) were evenly distributed. Microbiological analysis (MRPP test, α- and β-diversities) showed that diversity of gut bacteria was significantly related to arsenic exposure through food, but diversity of gut fungi is less affected. Manhattan plot and LEfSe analysis showed that arsenic exposure significantly changes microbial taxa, which might be directly associated with arsenic metabolism and diseases mediated by arsenic exposure, such as Deltaproteobacteria, Polynucleobacter, Saccharomyces, Candida, Amanitaceae, and Fusarium. Network analysis was used to identify the changing hub taxa in feces along with arsenic exposure. Function predicting analysis indicated that arsenic exposure might also significantly increase differential metabolic pathways and would disturb carbohydrates, lipid, and amino acids metabolism of gut bacteria. Conclusions: The results demonstrate that subchronic arsenic exposure via food significantly changes the gut microbiome, and the toxicity of arsenic in food, especially in staples, should be comprehensively evaluated in terms of the disturbance of microbiome, and feces might be the main pathway through which arsenic from food exposure is excreted and bio-transformed, providing a new insight into the investigation of bio-detoxification for arseniasis. © 2020 The Authors
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被引频次[WOS]:6   [查看WOS记录]     [查看WOS中相关记录]
Document Type: 期刊论文
Appears in Collections:气候变化与战略

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Affiliation: Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, 523808, China; Nanchang Key Laboratory of Microbial Resources Exploitation & Utilization from Poyang Lake Wetland, Jiangxi Normal University, Nanchang, 330022, China; Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518000, China; Department of Toxicology, School of Public Health, Southern Medical University, 1023 S. Shatai Road, Guangzhou, 510515, China

Recommended Citation:
Wang J.,Hu W.,Yang H.,et al. Arsenic concentrations, diversity and co-occurrence patterns of bacterial and fungal communities in the feces of mice under sub-chronic arsenic exposure through food[J]. Environment International,2020-01-01,138
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