DOI: 10.1016/j.scitotenv.2020.136838
论文题名: Hydrological variations shape diversity and functional responses of streambed microbes
作者: Gionchetta G. ; Oliva F. ; Romaní A.M. ; Bañeras L.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 714 语种: 英语
英文关键词: DNA-RNA Illumina sequencing
; Functional microbial diversity
; Streambed biofilms
; Water stress
Scopus关键词: Data streams
; DNA
; Driers (materials)
; Ecosystems
; Enzymes
; Gene encoding
; Microorganisms
; Physiology
; RNA
; Community level physiological profiles
; Environmental conditions
; Extracellular enzyme activity
; Illumina
; Microbial diversity
; Microbial functional diversities
; Streambeds
; Water stress
; Physiological models
; biofilm
; DNA
; ecosystem function
; environmental conditions
; functional response
; hydrology
; microbial community
; microorganism
; RNA
; stream bed
; water stress
; climate change
; desiccation
; genomics
; hydrological change
; species diversity
; acclimatization
; article
; biofilm
; DNA RNA hybridization
; drought
; enzyme activity
; eukaryote
; functional assessment
; illumina sequencing
; microbial biomass
; microbial community
; microbial diversity
; nonhuman
; prokaryote
; turnover rate
; water stress
; Eukaryota
; Prokaryota
英文摘要: Microbiota inhabiting the intermittent streambeds mediates several in-stream processes that are essential for ecosystem function. Reduced stream discharge caused by the strengthened intermittency and increased duration of the dry phase is a spreading global response to changes in climate. Here, the impacts of a 5-month desiccation, one-week rewetting and punctual storms, which interrupted the dry period, were examined. The genomic composition of total (DNA) and active (RNA) diversity, and the community level physiological profiles (CLPP) were considered as proxies for functional diversity to describe both prokaryotes and eukaryotes inhabiting the surface and hyporheic streambeds. Comparisons between the genomic and potential functional responses helped to understand how and whether the microbial diversity was sensitive to the environmental conditions and resource acquisition, such as water stress and extracellular enzyme activities, respectively. RNA expression showed the strongest relationship with the environmental conditions and resource acquisition, being more responsive to changing conditions compared to DNA diversity, especially in the case of prokaryotes. The DNA results presumably reflected the legacy of the treatments because inactive, dormant, or dead cells were included, suggesting a slow microbial biomass turnover or responses of the microbial communities to changes mainly through physiological acclimation. On the other hand, microbial functional diversity was largely explained by resources acquisition, such as metrics of extracellular enzymes, and appeared vulnerable to the hydrological changes and duration of desiccation. The data highlight the need to improve the functional assessment of stream ecosystems with the application of complementary metrics to better describe the streambed microbial dynamics under dry-rewet stress. © 2018 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158359
Appears in Collections: 气候变化与战略
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作者单位: GRECO, Institute of Aquatic Ecology, University of Girona, Girona, 17003, Spain; Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain; Molecular Microbial Ecology Group, Institute of Aquatic Ecology, University of Girona, Girona, 17003, Spain
Recommended Citation:
Gionchetta G.,Oliva F.,Romaní A.M.,et al. Hydrological variations shape diversity and functional responses of streambed microbes[J]. Science of the Total Environment,2020-01-01,714