globalchange  > 气候变化与战略
DOI: 10.1016/j.scitotenv.2019.135200
论文题名:
Molecular mechanisms underlying the effects of temperature increase on Mytilus sp. and their hybrids at early larval stages
作者: Mlouka R.; Cachot J.; Sforzini S.; Oliveri C.; Boukadida K.; Clerandeau C.; Pacchioni B.; Millino C.; Viarengo A.; Banni M.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 708
语种: 英语
英文关键词: Global changes ; Immunhistochemistry hybrids ; Mytilus sp. ; Temperature ; Transcriptomic
Scopus关键词: Amino acids ; Ecosystems ; Metabolism ; Proteins ; Effects of temperature ; Global change ; Histidine-rich glycoproteins ; Immunhistochemistry hybrids ; Immunohistochemistry ; Mytilus sp ; Oxidative stress response ; Transcriptomic ; Temperature ; experimental study ; gene expression ; global change ; larval development ; molecular analysis ; physiological response ; protein ; shell ; temperature effect ; toxicity ; Mytilus ; Mytilus edulis ; Mytilus sp.
英文摘要: The present work aims to investigate the effects of water temperature increase on Mytilus galloprovincilis and Mytilus edulis pure larvae (PG, PE) and their hybrids (HFG, HFE). D-larvae were maintained at 18 °C or exposed to a higher temperature of 22 °C for 48 h. Initially, Embryotoxicity test was evaluated. Second, a transcriptomic analysis using a recently developed microarray platform was applied to determine the main biological processes involved in early life stages responses to temperature increase. Finally, an immunofluorescence investigation was performed to bridge the gap between transcriptomic regulation and the real changes at cellular/tissue levels. Embryotoxicity test revealed a higher sensitivity of M. edulis (PE) D-larvae as well as hybrids from females M. edulis (HFE) to temperature increase, with the highest rate of larval malformations. Transcriptomic results indicated a lack of an adequate heat shock protein (Hsp) response in PE and HFE larvae (the high expression was observed in PG larvae); the differential expression of gene involved in translation, energy metabolism and oxidative stress response may contribute to explain the observed complex alterations in the studied conditions. As revealed by immunohistochemistry, cytoskeleton proteins changes associated with a drastic decrease of Histidine-Rich Glycoprotein (HRG) may elucidate the larval abnormalities in shell development observed for PE and HFE larvae. Overall, the results indicate that each type of pure larva (PG and PE) and their respective female hybrid (HFG and HFE) react similarly to the temperature increase. Our data should be carefully considered in view of the water temperature increase in marine ecosystems and especially for the mussel's species in confluence zones. © 2019 Elsevier B.V.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158683
Appears in Collections:气候变化与战略

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作者单位: Laboratory of Oceanic and Continental Environments and Paleoenvironments, University of Bordeaux, EPOC, UMR 5805, Pessac, F-33600, France; Laboratory of Biochemistry and Environmental, ISA, Chott-Mariem, Sousse, 4042, Tunisia; Higher Institute of Biotechnology of Monastir.5000, Monastir University, Tunisia; Department of Sciences and Technological Innovation (DiSIT), University of Piemonte Orientale “A. Avogadro”, V.le T. Michel 11, Alessandria, 15121, Italy; Dipartimento di Scienze della terra, dell'ambiente e della vita (DISTAV), Università degli Studi di Genova, Via Balbi 5, Genova, 16126, Italy; CRIBI Biotechnology Center, University of Padova, Padova, 35131, Italy; Laboratory of Environmental Chemistry and Toxicology, IRCCS - Istituto di Ricerche Farmacologiche Mario Negri, Via Mario Negri 2, Milano, 20156, Italy

Recommended Citation:
Mlouka R.,Cachot J.,Sforzini S.,et al. Molecular mechanisms underlying the effects of temperature increase on Mytilus sp. and their hybrids at early larval stages[J]. Science of the Total Environment,2020-01-01,708
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