DOI: 10.1016/j.aquaculture.2019.734830
论文题名: Characterizing transcriptome changes in gill tissue of turbot (Scophthalmus maximus) for waterless preservation
作者: Nie X. ; Zhang C. ; Jiang C. ; Li S. ; Hong W. ; Chen S. ; Zhang Y.
刊名: Aquaculture
ISSN: 448486
出版年: 2020
卷: 518 语种: 英语
英文关键词: Gene expression
; RNA-seq
; Scophthalmus maximus
; Transcriptome
Scopus关键词: demersal fish
; gene expression
; homeostasis
; low temperature
; metabolism
; oxidative stress
; physiological response
; RNA
; Scophthalmidae
; Scophthalmus maximus
英文摘要: The combined method with high oxygen filling under low temperature and waterless condition is suggested to be a more effective strategy to keep fish alive during turbot transportation. To investigate the biological mechanism of turbot, when turbot was subjected to waterless treatment under different oxygen conditions (water, air and oxygen), the global expression profiling of the gill tissues was performed by transcriptome analysis using Illumina HiSeq 4000 platform. The results showed that a total of 104,886 unigenes were detected, and differential expression analysis revealed 662 differentially expressed genes (322 upregulated (fold >2) and 340 downregulated (fold <0.5)) in the Air-group, and 244 up-regulated and 197 down-regulated genes in the Oxygen-group in comparison with the control (in water at 2 °C), and 354 up-regulated and 212 down-regulated genes in the Oxygen-group compared to the Air-group were identified. In addition, the reliability of the sequencing results was confirmed using qPCR, the fold change of 6 randomly selected genes were consistent with the results of the sequencing. Moreover, gene ontology analysis was conducted, which indicated that the air environment with waterless has a greater impact on the metabolism of fish compared with the water environment. It was supposed that turbot may regulate their metabolism by increasing the expression of genes involved in transcription and promoting the oxidative stress response in order to maintain homoeostasis. Furthermore, GO and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the differentially expressed genes were enriched in lyase activity, immune response, cytokine activity and nitrogen metabolism, revealing that oxygen availability influences above metabolic activities in response to waterless conditions. This study provides a novel insight into the mechanism of physiological changes of turbot during waterless transportation. © 2019 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158696
Appears in Collections: 气候变化与战略
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作者单位: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China; Shandong Key Laboratory of Storage and Transportation Technology of Agricultural Products, Jinan, 250103, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China
Recommended Citation:
Nie X.,Zhang C.,Jiang C.,et al. Characterizing transcriptome changes in gill tissue of turbot (Scophthalmus maximus) for waterless preservation[J]. Aquaculture,2020-01-01,518