DOI: 10.1016/j.scitotenv.2019.135929
论文题名: Taxonomy and functional interactions in upper and bottom waters of an oligotrophic high-mountain deep lake (Redon, Pyrenees) unveiled by microbial metagenomics
作者: Llorens-Marès T. ; Catalan J. ; Casamayor E.O.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 707 语种: 英语
英文关键词: Alpine lake
; Bacteria
; Biogeochemistry
; Functional genes
; Microbial biodiversity
Scopus关键词: Ammonia
; Bacteria
; Biodiversity
; Biogeochemistry
; Carbon monoxide
; Genes
; Ionization of gases
; Landforms
; Nitrogen fixation
; Oxidation
; RNA
; Sulfur
; Alpine lakes
; Biogeochemical interactions
; Biogeochemical process
; Carbon monoxide oxidation
; Environmental conditions
; Functional genes
; Global environmental change
; Microbial biodiversity
; Lakes
; carbon
; nitrogen
; phosphorus
; RNA 16S
; RNA 18S
; sulfur
; alpine environment
; bacterium
; biodiversity
; biogeochemistry
; bottom water
; genomics
; oligotrophic environment
; taxonomy
; Actinobacteria
; aerobic metabolism
; anaerobic ammonium oxidation
; Article
; atmospheric deposition
; autumn
; Burkholderiales
; carbon cycling
; controlled study
; denitrification
; DNA sequence
; hidden Markov model
; lake
; metagenomics
; methanogenesis
; microbial biomass
; microbial diversity
; microbial genetics
; mineralization
; nitrification
; nitrogen cycling
; nitrogen fixation
; nonhuman
; phosphorus cycle
; population abundance
; priority journal
; spring
; sulfur cycle
; taxonomy
; vegetation
; Catalonia
; Lake Redon
; Lerida [Catalonia]
; Spain
; Actinobacteria
; Burkholderiales
英文摘要: High mountain lakes are, in general, highly sensitive systems to external forcing and good sentinels of global environmental changes. For a better understanding of internal lake processes, we examined microbial biodiversity and potential biogeochemical interactions in the oligotrophic deep high-mountain Lake Redon (Pyrenees, 2240 m altitude) using shotgun metagenomics. We analyzed the two ends of the range of environmental conditions found in Lake Redon, at 2 and 60 m depths. Bacteria were the most abundant component of the metagenomic reads (>90%) and the diversity indices of both taxonomic (16S and 18S rRNA) and functional (carbon-, nitrogen-, sulfur-, and phosphorous-cycling) related genes were higher in the bottom dark layer than in the upper compartment. A marked segregation was observed both in biodiversity and in the dominant energy and biomass generating pathways between the extremes. The aerobic respiration was mainly dominated by heterotrophic Burkholderiales at the top and Actinobacteria and Burkholderiales at the lake bottom. The potential for an active nitrogen cycle (nitrogen fixation, nitrification, nitrite oxidation, and nitrate reduction) was mainly found at 60 m, and potential for methanogenesis, anaerobic ammonia oxidation and dissimilatory sulfur pathways were only observed there. Some unexpected and mostly unseen energy and biomass pathways were found relevant for the biogeochemical cycling in lake Redon, i.e., those related to carbon monoxide oxidation and phosphonates processing. We provide a general scheme of the main biogeochemical processes that may operate in the sentinel deep Lake Redon. This framework may help for a better understanding of the whole lake metabolism. © 2019 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158726
Appears in Collections: 气候变化与战略
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作者单位: Integrative Freshwater Ecology Group, Center for Advanced Studies of Blanes-CSIC, Acc. Cala St Francesc 14, Blanes, Catalonia E-17300, Spain; CREAF - CSIC, Campus UAB, Edifici C, Cerdanyola del Vallès, Catalonia 08193, Spain
Recommended Citation:
Llorens-Marès T.,Catalan J.,Casamayor E.O.. Taxonomy and functional interactions in upper and bottom waters of an oligotrophic high-mountain deep lake (Redon, Pyrenees) unveiled by microbial metagenomics[J]. Science of the Total Environment,2020-01-01,707