DOI: 10.1039/c9an02032b
论文题名: Global metabolomics analysis of serum from humans at risk of thrombotic stroke
作者: Khan A. ; Shin M.-S. ; Jee S.H. ; Park Y.H.
刊名: Analyst
ISSN: 32654
出版年: 2020
卷: 145, 期: 5 语种: 英语
英文摘要: We aimed to determine the serum concentrations of altered compounds to understand the changes in metabolism and pathophysiology that occur prior to thrombotic stroke. In this prospective cohort study, high-resolution metabolomics (HRM) was employed to analyze serum samples obtained from patients at risk of stroke (n = 99) and non-risk controls (n = 301). Partial least-squares discriminant analysis (PLS-DA), along with univariate analysis using a false discovery rate (FDR) of q = 0.05 were employed to identify the discriminant metabolic profiles and to determine significantly different metabolites between healthy control and stroke risk groups. PLS-DA satisfactorily separated the stroke risk sera from control sera. Additionally, these discriminant metabolic profiles were not related to hypertension, smoking, diabetes mellitus, or insulin sensitivity. A group of 35 metabolites, most of them amino acids, that were capable of discriminating stroke risk sera from controls were identified using untargeted metabolomics. Further, the targeted metabolomics approach confirmed that the quantified concentrations of l-tryptophan, 3-methoxytyramine, methionine, homocysteinesulfinic acid, cysteine, isoleucine, carnitine, arginine, linoleic acid, and sphingosine were specifically elevated in the sera of patients who were later diagnosed with stroke. Our untargeted and targeted metabolomics approaches support investigating these compounds as novel biomarkers for early and non-invasive detection of thrombotic stroke. © The Royal Society of Chemistry 2020. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158736
Appears in Collections: 气候变化与战略
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作者单位: Metabolomics Laboratory, Korea University College of Pharmacy, Sejong, 30019, South Korea; School of Global Sport Studies, Korea University, Sejong, 30019, South Korea; Department of Epidemiology and Health Promotion, Institute for Health Promotion, Graduate School of Public Health, Yonsei University, Seoul, 03722, South Korea
Recommended Citation:
Khan A.,Shin M.-S.,Jee S.H.,et al. Global metabolomics analysis of serum from humans at risk of thrombotic stroke[J]. Analyst,2020-01-01,145(5)