DOI: 10.1016/j.ecoenv.2019.110116
论文题名: Comparative transcriptome analysis reveals Dusp1 as a critical regulator of inflammatory response to fly ash particle exposure in mouse
作者: Zhang J. ; Chen Y. ; Namani A. ; Elshaer M. ; Jiang Z. ; Shi H. ; Tang X. ; Wang X.J.
刊名: Ecotoxicology and Environmental Safety
ISSN: 1476513
出版年: 2020
卷: 190 语种: 英语
英文关键词: Dusp1
; Fly ash particle
; Inflammation
; Lung
; RNA-Seq profiling
Scopus关键词: interleukin 6
; mitogen activated protein kinase phosphatase 1
; transcriptome
; comparative study
; concentration (composition)
; fly ash
; gene expression
; health risk
; incineration
; particulate matter
; pollution exposure
; respiratory disease
; RNA
; animal experiment
; animal model
; animal tissue
; Article
; China
; controlled study
; fly ash
; gene mapping
; gene ontology
; genetic code
; genotype
; immune response
; male
; mouse
; municipal waste
; nonhuman
; particulate matter
; pneumonia
; protein blood level
; RNA analysis
; RNA sequencing
; signal transduction
; transcriptomics
; transmission electron microscopy
; China
; Mus
英文摘要: Exposure to outdoor concentrations of fine particulate matter (PM2.5) is a leading global health concern. Waste incineration emission has been recognized as a potential major contributor of ambient PM2.5. Respiratory inflammation is a central feature induced by PM2.5 exposure by inhalation. However, the molecular mechanisms are not fully understood. Dual-specificity phosphatase 1 (Dusp1) plays an instrumental role in the regulation of airway inflammation. In this study, fly ash particles (20 mg/kg BW) collected from a municipal waste incinerator in China were given to BALB/c wild-type (WT) and Dusp1−/− mice by intranasal administration daily for three consecutive days. While these particles induced mild inflammation in both genotypes, a significantly higher level of serum interleukin-6 (665 pg/ml) was measured in Dusp1−/− mice challenged with fly ash particles than in their WT counterparts. Genome-wide transcriptome profiling of pulmonary coding genes in response to the exposure were performed in both genotypes by RNA sequencing. We identified 487 differentially-expressed genes (DEGs) in fly ash-challenged Dusp1−/− mice versus their WT counterparts with a log2fold-change >1.5 and p < 0.05. Functional enrichment and molecular pathway mapping of the DEGs specific to Dusp1−/− mice exposed to the particles revealed that the top 10 perturbed molecular pathways were associated with the immune response. Our study demonstrates the anti-inflammatory role of Dusp1 in protecting the lung against insults by fly ash particles, suggesting that Dusp1 might be a therapeutic target for the treatment of PM2.5-induced respiratory diseases. © 2019 Elsevier Inc. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159160
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: Department of Pharmacology and Cancer Institute of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China; Department of Biochemistry and Department of Thoracic Surgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China; Department of Pathology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China; The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, 322000, China
Recommended Citation:
Zhang J.,Chen Y.,Namani A.,et al. Comparative transcriptome analysis reveals Dusp1 as a critical regulator of inflammatory response to fly ash particle exposure in mouse[J]. Ecotoxicology and Environmental Safety,2020-01-01,190