DOI: 10.1016/j.watres.2019.115277
论文题名: Metagenomic analysis of β-lactamase and carbapenemase genes in the wastewater resistome
作者: Makowska N. ; Philips A. ; Dabert M. ; Nowis K. ; Trzebny A. ; Koczura R. ; Mokracka J.
刊名: Water Research
ISSN: 431354
出版年: 2020
卷: 170 语种: 英语
英文关键词: Antibiotic resistance
; Coliforms
; CPE
; ESBL
; Escherichia coli
Scopus关键词: Antibiotics
; Biodiversity
; Effluent treatment
; Effluents
; Escherichia coli
; Genes
; Health risks
; Public health
; Reclamation
; Sewage
; Sewage aeration
; Antibiotic resistance
; Antibiotic-resistant bacteria
; Central wastewater treatment plants
; Coliforms
; ESBL
; Resistance to antibiotics
; Wastewater disinfection
; Wastewater treatment process
; Wastewater treatment
; beta lactamase
; carbapenemase
; carbapenemase bla ges
; carbapenemase bla ndm
; carbapenemase bla oxa 48
; carbapenemase bla vim
; extended spectrum beta lactamase
; extended spectrum beta lactamase bla ctx m
; unclassified drug
; antiinfective agent
; bacterial protein
; beta lactamase
; carbapenemase
; antibiotic resistance
; antibiotics
; bacterium
; biodiversity
; coliform bacterium
; effluent
; enzyme
; enzyme activity
; genome
; genomics
; microbial activity
; microbial community
; relative abundance
; wastewater
; wastewater treatment
; wastewater treatment plant
; aeration
; antibiotic resistome
; Article
; bacterial genome
; effluent
; gene frequency
; metagenome
; metagenomics
; microbial diversity
; nonhuman
; Poland
; priority journal
; qualitative analysis
; quantitative analysis
; sewage
; waste water
; waste water management
; metagenome
; Poland [Central Europe]
; Bacteria (microorganisms)
; Escherichia coli
; Anti-Bacterial Agents
; Bacterial Proteins
; beta-Lactamases
; Metagenome
; Poland
; Waste Water
英文摘要: The emergence and spread of resistance to antibiotics among bacteria is the most serious global threat to public health in recent and coming decades. In this study, we characterized qualitatively and quantitatively β-lactamase and carbapenemase genes in the wastewater resistome of Central Wastewater Treatment Plant in Koziegłowy, Poland. The research concerns determination of the frequency of genes conferring resistance to β-lactam and carbapenem antibiotics in the genomes of culturable bacteria, as well as in the wastewater metagenome at three stages of treatment: raw sewage, aeration tank, and final effluent. In the final effluent we found bacteria with genes that pose the greatest threat to public health, including genes of extended spectrum β-lactamases – blaCTX-M, carbapenemases – blaNDM, blaVIM, blaGES, blaOXA-48, and showed that during the wastewater treatment their frequency increased. Moreover, the wastewater treatment process leads to significant increase in the relative abundance of blaTEM and blaGES genes and tend to increase the relative abundance of blaCTX-M, blaSHV and blaOXA-48 genes in the effluent metagenome. The biodiversity of bacterial populations increased during the wastewater treatment and there was a correlation between the change in the composition of bacterial populations and the variation of relative abundance of β-lactamase and carbapenemase genes. PCR-based quantitative metagenomic analysis combined with analyses based on culture methods provided significant information on the routes of ARBs and ARGs spread through WWTP. The limited effectiveness of wastewater treatment processes in the elimination of antibiotic-resistant bacteria and resistance genes impose the need to develop an effective strategy and implement additional methods of wastewater disinfection, in order to limit the increase and the spread of antibiotic resistance in the environment. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159167
Appears in Collections: 气候变化与战略
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作者单位: Department of Microbiology, Faculty of Biology, Adam Mickiewicz University in Poznań, Poznań, Poland; European Center for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland; Molecular Biology Techniques Laboratory, Faculty of Biology, Adam Mickiewicz University in Poznań, Poznań, Poland
Recommended Citation:
Makowska N.,Philips A.,Dabert M.,et al. Metagenomic analysis of β-lactamase and carbapenemase genes in the wastewater resistome[J]. Water Research,2020-01-01,170