DOI: 10.1093/jxb/erz530
论文题名: Chronic exposure of soybean plants to nanomolar cadmium reveals specific additional high-affinity targets of cadmium toxicity
作者: Andresen E. ; Lyubenova L. ; Hubáček T. ; Bokhari S.N.H. ; Matoušková Š. ; Mijovilovich A. ; Rohovec J. ; Küpper H.
刊名: Journal of experimental botany
ISSN: 14602431
出版年: 2020
卷: 71, 期: 4 语种: 英语
英文关键词: Cadmium
; lipidomics
; metabolomics
; metal stress
; metalloproteomics
; soybean (Glycine max)
; sublethal toxicity
; XANES
英文摘要: Solving the global environmental and agricultural problem of chronic low-level cadmium (Cd) exposure requires better mechanistic understanding. Here, soybean (Glycine max) plants were exposed to Cd concentrations ranging from 0.5 nM (background concentration, control) to 3 µM. Plants were cultivated hydroponically under non-nodulating conditions for 10 weeks. Toxicity symptoms, net photosynthetic oxygen production and photosynthesis biophysics (chlorophyll fluorescence: Kautsky and OJIP) were measured in young mature leaves. Cd binding to proteins [metalloproteomics by HPLC-inductively coupled plasma (ICP)-MS] and Cd ligands in light-harvesting complex II (LHCII) [X-ray absorption near edge structure (XANES)], and accumulation of elements, chloropyll, and metabolites were determined in leaves after harvest. A distinct threshold concentration of toxicity onset (140 nM) was apparent in strongly decreased growth, the switch-like pattern for nutrient uptake and metal accumulation, and photosynthetic fluorescence parameters such as Φ RE10 (OJIP) and saturation of the net photosynthetic oxygen release rate. XANES analyses of isolated LHCII revealed that Cd was bound to nitrogen or oxygen (and not sulfur) atoms. Nutrient deficiencies caused by inhibited uptake could be due to transporter blockage by Cd ions. The changes in specific fluorescence kinetic parameters indicate electrons not being transferred from PSII to PSI. Inhibition of photosynthesis combined with inhibition of root function could explain why amino acid and carbohydrate metabolism decreased in favour of molecules involved in Cd stress tolerance (e.g. antioxidative system and detoxifying ligands). © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159243
Appears in Collections: 气候变化与战略
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作者单位: Biology Centre, Institute of Plant Molecular Biology, Department of Plant Biophysics and Biochemistry. Budějovice, Czech Academy of Sciences, Czech Republic; Biology Centre, Institute of Hydrobiology, Department of Hydrochemistry and Ecosystem Modelling, Czech Academy of Sciences, Czech Republic; Institute of Geology, Department of Geological Processes, Czech Academy of SciencesPraha, Czech Republic; University of South Bohemia, Faculty of Sciences, Department of Experimental Plant Biology, České Budějovice, Czech Republic
Recommended Citation:
Andresen E.,Lyubenova L.,Hubáček T.,et al. Chronic exposure of soybean plants to nanomolar cadmium reveals specific additional high-affinity targets of cadmium toxicity[J]. Journal of experimental botany,2020-01-01,71(4)