DOI: 10.1021/acs.jafc.9b04887
论文题名: Comparative Proteomic Analysis Reveals the Molecular Mechanisms Underlying the Accumulation Difference of Bioactive Constituents in Glycyrrhiza uralensis Fisch under Salt Stress
作者: Wang C. ; Chen L. ; Cai Z.C. ; Chen C. ; Liu Z. ; Liu X. ; Zou L. ; Chen J. ; Tan M. ; Wei L. ; Mei Y.
刊名: Journal of Agricultural and Food Chemistry
ISSN: 218561
出版年: 2020
卷: 68, 期: 5 语种: 英语
英文关键词: bioactive constituents
; Glycyrrhiza uralensis Fisch
; proteomics
; salt stress
Scopus关键词: Amino acids
; Ammonia
; Biochemistry
; Biosynthesis
; Enzymes
; Flavonoids
; Flavor compounds
; International trade
; Proteomics
; bioactive constituents
; Biosynthesis pathways
; Chalcone flavanone isomerase
; Expression patterns
; Glycyrrhiza uralensis Fisch
; Molecular mechanism
; Salt stresss
; Structural proteins
; Plants (botany)
英文摘要: Licorice (Glycyrrhiza uralensis Fisch) possesses a substantial share of the global markets for its unique sweet flavor and diverse pharmacological compounds. Cultivated licorice is widely distributed in northwest regions of China, covered with land with a broad range of salinities. A preliminary study indicated that suitable salt stress significantly increased the content of bioactive constituents in licorice. However, the molecular mechanisms underlying the influence of salinity on the accumulation of these constituents remain unclear, which hinders quality breeding of cultivated licorice. In our study, flavonoid-related structural genes were obtained, and most of them, such as phenylalanine ammonia-lyases, cinnamate 4-hydroxylases, 4-coumarate: CoA ligases, chalcone synthases, chalcone-flavanone isomerase, and flavonol synthase, showed high levels after salt treatment. In the biosynthesis of glycyrrhizin, three key enzymes (bAS, CYP88D6, and CYP72A154) were identified as differentially expressed proteins and remarkably upregulated in the salt-stressed group. Combining these results with the contents of 14 bioactive constituents, we also found that the expression patterns of those structural proteins were logically consistent with changes in bioactive constituent profiles. Thus, we believe that suitable salt stress increased the accumulation of bioactive constituents in licorice by upregulating proteins involved in the related biosynthesis pathways. This work provided valuable proteomic information for unraveling the molecular mechanism of flavonoid and glycyrrhizin metabolism and offered fundamental resources for quality breeding in licorice. © 2020 American Chemical Society. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159369
Appears in Collections: 气候变化与战略
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作者单位: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, 210023, China; Natl. and Loc. Collab. Eng. Ctr. of Chinese Med. Rsrc. Industrialization and Formulae Innov. Med., Nanjing, 210023, China
Recommended Citation:
Wang C.,Chen L.,Cai Z.C.,et al. Comparative Proteomic Analysis Reveals the Molecular Mechanisms Underlying the Accumulation Difference of Bioactive Constituents in Glycyrrhiza uralensis Fisch under Salt Stress[J]. Journal of Agricultural and Food Chemistry,2020-01-01,68(5)