globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1218661109
论文题名:
Gene expression changes governing extreme dehydration tolerance in an Antarctic insect
作者: Teets N.M.; Peyton J.T.; Colinet H.; Renault D.; Kelley J.L.; Kawarasaki Y.; Lee R.E.; Jr.; Denlinger D.L.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2012
卷: 109, 期:50
起始页码: 20744
结束页码: 20749
语种: 英语
英文关键词: Environmental stress ; Physiological ecology
Scopus关键词: adenosine triphosphate ; cytochrome c ; proteasome ; ubiquitin ; primer DNA ; Antarctica ; Article ; autophagy ; Belgica antarctica ; body water ; controlled study ; dehydration ; down regulation ; environmental stress ; gene expression ; insect ; Megaphorura arctica ; next generation sequencing ; nonhuman ; oxidation reduction reaction ; priority journal ; proton transport ; RNA sequence ; transcriptomics ; upregulation ; water loss ; acclimatization ; animal ; arthropod ; article ; Chironomidae ; comparative study ; dehydration ; gene ; gene expression ; genetics ; metabolome ; nucleotide sequence ; pathophysiology ; physiology ; species difference ; Acclimatization ; Animals ; Antarctic Regions ; Arthropods ; Base Sequence ; Chironomidae ; Dehydration ; DNA Primers ; Gene Expression ; Genes, Insect ; Metabolome ; Species Specificity
英文摘要: Among terrestrial organisms, arthropods are especially susceptible to dehydration, given their small body size and high surface area to volume ratio. This challenge is particularly acute for polar arthropods that face near-constant desiccating conditions, as water is frozen and thus unavailable for much of the year. The molecular mechanisms that govern extreme dehydration tolerance in insects remain largely undefined. In this study, we used RNA sequencing to quantify transcriptional mechanisms of extreme dehydration tolerance in the Antarctic midge, Belgica antarctica, the world’s southernmost insect and only insect endemic to Antarctica. Larvae of B. antarctica are remarkably tolerant of dehydration, surviving losses up to 70% of their body water. Gene expression changes in response to dehydration indicated up-regulation of cellular recycling pathways including the ubiquitin-mediated proteasome and autophagy, with concurrent down-regulation of genes involved in general metabolism and ATP production. Metabolomics results revealed shifts in metabolite pools that correlated closely with changes in gene expression, indicating that coordinated changes in gene expression and metabolism are a critical component of the dehydration response. Finally, using comparative genomics, we compared our gene expression results with a transcriptomic dataset for the Arctic collembolan, Megaphorura arctica. Although B. antarctica and M. arctica are adapted to similar environments, our analysis indicated very little overlap in expression profiles between these two arthropods. Whereas several orthologous genes showed similar expression patterns, transcriptional changes were largely species specific, indicating these polar arthropods have developed distinct transcriptional mechanisms to cope with similar desiccating conditions. © 2012, National Academy of Sciences. All rights reserved.
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被引频次[WOS]:91   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/162224
Appears in Collections:气候变化与战略

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作者单位: Teets, N.M., Department of Entomology, Ohio State University, Columbus, OH 43210, United States; Peyton, J.T., Department of Evolution, Ecology, and Organismal Biology, Ohio State University, Columbus, OH 43210, United States; Colinet, H., Unité Mixte de Recherche, Centre National de la Recherche Scientifique 6553 Ecobio, Université de Rennes 1, Rennes Cedex, 35042, France, Earth and Life Institute, Biodiversity Research Centre (BDIV), Catholic University of Louvain, Louvain-la-Neuve, B-1348, Belgium; Renault, D., Unité Mixte de Recherche, Centre National de la Recherche Scientifique 6553 Ecobio, Université de Rennes 1, Rennes Cedex, 35042, France; Kelley, J.L., Department of Genetics, Stanford University, Stanford, CA 94305, United States; Kawarasaki, Y., Department of Zoology, Miami University, Oxford, OH 45056, United States; Lee, R.E., Jr., Department of Zoology, Miami University, Oxford, OH 45056, United States; Denlinger, D.L., Department of Entomology, Ohio State University, Columbus, OH 43210, United States, Department of Evolution, Ecology, and Organismal Biology, Ohio State University, Columbus, OH 43210, United States

Recommended Citation:
Teets N.M.,Peyton J.T.,Colinet H.,et al. Gene expression changes governing extreme dehydration tolerance in an Antarctic insect[J]. Proceedings of the National Academy of Sciences of the United States of America,2012-01-01,109(50)
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