DOI: 10.1073/pnas.0708183104
论文题名: Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals
作者: Rohde R.A. ; Price P.B.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2007
卷: 104, 期: 42 起始页码: 16592
结束页码: 16597
语种: 英语
英文关键词: Dissolved molecules
; F420 fluorescence
; Fluorimetry
; Ice cores
; Single cells
Scopus关键词: article
; cell isolation
; diffusion
; fluorescence
; fluorometry
; grain
; mathematical computing
; microbial activity
; microbial growth
; microorganism
; nonhuman
; oxidation reduction reaction
; priority journal
; roentgen spectroscopy
; scanning electron microscopy
英文摘要: Two known habitats for microbial metabolism in ice are surfaces of mineral grains and liquid veins along three-grain boundaries. We propose a third, more general, habitat in which a microbe frozen in ice can metabolize by redox reactions with dissolved small molecules such as CO2, O2, N2, CO, and CH4 diffusing through the ice lattice. We show that there is an adequate supply of diffusing molecules throughout deep glacial ice to sustain metabolism for >105 yr. Using scanning fluorimetry to map proteins (a proxy for cells) and F420 (a proxy for methanogens) in ice cores, we find isolated spikes of fluorescence with intensity consistent with as few as one microbial cell in a volume of 0.16 μl with the protein mapper and in 1.9 μl with the methanogen mapper. With such precise localization, it should be possible to extract single cells for molecular identification. © 2007 by The National Academy of Sciences of the USA. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/162466
Appears in Collections: 气候变化与战略
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作者单位: Rohde, R.A., Department of Physics, University of California, Berkeley, CA 94720, United States; Price, P.B., Department of Physics, University of California, Berkeley, CA 94720, United States
Recommended Citation:
Rohde R.A.,Price P.B.. Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals[J]. Proceedings of the National Academy of Sciences of the United States of America,2007-01-01,104(42)