globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2002635118
论文题名:
Control of septum thickness by the curvature of sepf polymers
作者: Wenzel M.; Celik Gulsoy I.N.; Gao Y.; Teng Z.; Willemse J.; Middelkamp M.; van Rosmalen M.G.M.; Larsen P.W.B.; van der Wel N.N.; Wuite G.J.L.; Roos W.H.; Hamoen L.W.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2021
卷: 118, 期:2
语种: 英语
英文关键词: Bacillus subtilis ; Cell division ; FtsZ ; SepF
英文摘要: Gram-positive bacteria divide by forming a thick cross wall. How the thickness of this septal wall is controlled is unknown. In this type of bacteria, the key cell division protein FtsZ is anchored to the cell membrane by two proteins, FtsA and/or SepF. We have isolated SepF homologs from different bacterial species and found that they all polymerize into large protein rings with diameters varying from 19 to 44 nm. Interestingly, these values correlated well with the thickness of their septa. To test whether ring diameter determines septal thickness, we tried to construct different SepF chimeras with the purpose to manipulate the diameter of the SepF protein ring. This was indeed possible and confirmed that the conserved core domain of SepF regulates ring diameter. Importantly, when SepF chimeras with different diameters were expressed in the bacterial host Bacillus subtilis, the thickness of its septa changed accordingly. These results strongly support a model in which septal thickness is controlled by curved molecular clamps formed by SepF polymers attached to the leading edge of nascent septa. This also implies that the intrinsic shape of a protein polymer can function as a mold to shape the cell wall. © 2021 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163347
Appears in Collections:气候变化与战略

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作者单位: Wenzel, M., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Celik Gulsoy, I.N., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Gao, Y., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Teng, Z., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Willemse, J., Molecular Biotechnology, Institute of Biology, Leiden University, Leiden, 2333 BE, Netherlands; Middelkamp, M., Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, 9747 AG, Netherlands; van Rosmalen, M.G.M., Department of Physics and Astronomy, Laser Lab., Free University of Amsterdam, Amsterdam, 1081 HV, Netherlands; Larsen, P.W.B., Department of Medical Biology, Electron Microscopy Center Amsterdam, Amsterdam UMC, Amsterdam, 1105 AZ, Netherlands; van der Wel, N.N., Department of Medical Biology, Electron Microscopy Center Amsterdam, Amsterdam UMC, Amsterdam, 1105 AZ, Netherlands; Wuite, G.J.L., Department of Physics and Astronomy, Laser Lab., Free University of Amsterdam, Amsterdam, 1081 HV, Netherlands; Roos, W.H., Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, 9747 AG, Netherlands; Hamoen, L.W., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands

Recommended Citation:
Wenzel M.,Celik Gulsoy I.N.,Gao Y.,et al. Control of septum thickness by the curvature of sepf polymers[J]. Proceedings of the National Academy of Sciences of the United States of America,2021-01-01,118(2)
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