DOI: 10.1073/pnas.2021166117
论文题名: Identification of signaling pathways; matrix-digestion enzymes; and motility components controlling Vibrio cholerae biofilm dispersal
作者: Bridges A.A. ; Fei C. ; Bassler B.L.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期: 51 起始页码: 32639
结束页码: 32647
语种: 英语
英文关键词: Biofilm dispersal
; Highcontent imaging
; Signal transduction
; Vibrio cholerae
; Virulence
Scopus关键词: adhesin
; polysaccharide
; Article
; bacterial virulence
; biofilm
; cell motility
; complex formation
; controlled study
; disease transmission
; gene identification
; nonhuman
; population dispersal
; priority journal
; Vibrio cholerae
英文摘要: Bacteria alternate between being free-swimming and existing as members of sessile multicellular communities called biofilms. The biofilm lifecycle occurs in three stages: cell attachment, biofilm maturation, and biofilm dispersal. Vibrio cholerae biofilms are hyperinfectious, and biofilm formation and dispersal are considered central to disease transmission. While biofilm formation is well studied, almost nothing is known about biofilm dispersal. Here, we conducted an imaging screen for V. cholerae mutants that fail to disperse, revealing three classes of dispersal components: signal transduction proteins, matrix-degradation enzymes, and motility factors. Signaling proteins dominated the screen and among them, we focused on an uncharacterized two-component sensory system that we term DbfS/DbfR for dispersal of biofilm sensor/regulator. Phospho-DbfR represses biofilm dispersal. DbfS dephosphorylates and thereby inactivates DbfR, which permits dispersal. Matrix degradation requires two enzymes: LapG, which cleaves adhesins, and RbmB, which digests matrix polysaccharides. Reorientation in swimming direction, mediated by CheY3, is necessary for cells to escape from the porous biofilm matrix. We suggest that these components act sequentially: signaling launches dispersal by terminating matrix production and triggering matrix digestion, and subsequent cell motility permits escape from biofilms. This study lays the groundwork for interventions aimed at modulating V. cholerae biofilm dispersal to ameliorate disease. © 2020 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163351
Appears in Collections: 气候变化与战略
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作者单位: Bridges, A.A., Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States, HHMI, Chevy Chase, MD 20815, United States; Fei, C., Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States; Bassler, B.L., Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States, HHMI, Chevy Chase, MD 20815, United States
Recommended Citation:
Bridges A.A.,Fei C.,Bassler B.L.. Identification of signaling pathways; matrix-digestion enzymes; and motility components controlling Vibrio cholerae biofilm dispersal[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(51)