globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2014659117
论文题名:
A bacterial cytolinker couples positioning of magnetic organelles to cell shape control
作者: Pfeiffer D.; Toro-Nahuelpan M.; Awal R.P.; Müller F.-D.; Bramkamp M.; Plitzko J.M.; Schüler D.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:50
起始页码: 32086
结束页码: 32097
语种: 英语
英文关键词: Cytoskeleton ; Magnetosome ; Magnetotaxis ; MamK ; MreB
Scopus关键词: actin ; binding protein ; curvature inducing coiled coil filament interacting with the magnetoskeleton ; cytoskeleton protein ; MamK protein ; MamY protein ; MreB protein ; unclassified drug ; Article ; ccfM gene ; cell division ; cell motility ; cell shape ; cell structure ; cellular distribution ; controlled study ; gene ; gene deletion ; gene overexpression ; magnetism ; magnetosome ; Magnetospirillum gryphiswaldense ; nonhuman ; pleiotropy ; priority journal ; protein motif
英文摘要: Magnetotactic bacteria maneuver within the geomagnetic field by means of intracellular magnetic organelles, magnetosomes, which are aligned into a chain and positioned at midcell by a dedicated magnetosome-specific cytoskeleton, the “magnetoskeleton.” However, how magnetosome chain organization and resulting magnetotaxis is linked to cell shape has remained elusive. Here, we describe the cytoskeletal determinant CcfM (curvature-inducing coiled-coil filament interacting with the magnetoskeleton), which links the magnetoskeleton to cell morphology regulation in Magnetospirillum gryphiswaldense. Membrane-anchored CcfM localizes in a filamentous pattern along regions of inner positive-cell curvature by its coiled-coil motifs, and independent of the magnetoskeleton. CcfM overexpression causes additional circumferential localization patterns, associated with a dramatic increase in cell curvature, and magnetosome chain mislocalization or complete chain disruption. In contrast, deletion of ccfM results in decreased cell curvature, impaired cell division, and predominant formation of shorter, doubled chains of magnetosomes. Pleiotropic effects of CcfM on magnetosome chain organization and cell morphology are supported by the finding that CcfM interacts with the magnetoskeleton-related MamY and the actin-like MamK via distinct motifs, and with the cell shape-related cytoskeleton via MreB. We further demonstrate that CcfM promotes motility and magnetic alignment in structured environments, and thus likely confers a selective advantage in natural habitats of magnetotactic bacteria, such as aquatic sediments. Overall, we unravel the function of a prokaryotic cytoskeletal constituent that is widespread in magnetic and nonmagnetic spirilla-shaped Alphaproteobacteria. © 2020 National Academy of Sciences. All rights reserved.
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被引频次[WOS]:7   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163354
Appears in Collections:气候变化与战略

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作者单位: Pfeiffer, D., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany; Toro-Nahuelpan, M., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany, Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Planegg-Martinsried, 82152, Germany; Awal, R.P., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany; Müller, F.-D., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany; Bramkamp, M., Institute for General Microbiology, Christian-Albrechts-University, Kiel, 24118, Germany; Plitzko, J.M., Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Planegg-Martinsried, 82152, Germany; Schüler, D., Department of Microbiology, University Bayreuth, Bayreuth, 95447, Germany

Recommended Citation:
Pfeiffer D.,Toro-Nahuelpan M.,Awal R.P.,et al. A bacterial cytolinker couples positioning of magnetic organelles to cell shape control[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(50)
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