globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1913177117
论文题名:
CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity
作者: Zhou Z.; Xu H.; Li Y.; Yang M.; Zhang R.; Shiraishi A.; Kiyonari H.; Liang X.; Huang X.; Wang Y.; Xie Q.; Liu S.; Chen R.; Bao L.; Guo W.; Wang Y.; Meng W.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:36
起始页码: 22193
结束页码: 22203
语种: 英语
英文关键词: CAMSAP1 ; Cell migration ; MARK2 ; Neuronal polarity ; Noncentrosomal microtubules
Scopus关键词: animal cell ; animal experiment ; animal tissue ; Article ; axon ; CAMSAP1 gene ; cell differentiation ; cell polarity ; controlled study ; gene ; homeostasis ; in vitro study ; in vivo study ; microtubule ; mouse ; nonhuman ; phenotype ; priority journal ; animal ; cell polarity ; drug effect ; gene expression regulation ; genetics ; immunoprecipitation ; metabolism ; microtubule ; nerve cell ; physiology ; Camsap1 protein, mouse ; microtubule associated protein ; paclitaxel ; protein binding ; Animals ; Cell Polarity ; Gene Expression Regulation ; Immunoprecipitation ; Mice ; Microtubule-Associated Proteins ; Microtubules ; Neurons ; Paclitaxel ; Protein Binding
英文摘要: The establishment of axon/dendrite polarity is fundamental for neurons to integrate into functional circuits, and this process is critically dependent on microtubules (MTs). In the early stages of the establishment process, MTs in axons change dramatically with the morphological building of neurons; however, how the MT network changes are triggered is unclear. Here we showthat CAMSAP1 plays a decisive role in the neuronal axon identification process by regulating the number of MTs. Neurons lacking CAMSAP1 form a multiple axon phenotype in vitro, while the multipolar-bipolar transition and radialmigration are blocked in vivo.We demonstrate that the polarity regulator MARK2 kinase phosphorylates CAMSAP1 and affects its ability to bind to MTs, which in turn changes the protection of MT minus-ends and also triggers asymmetric distribution of MTs. Our results indicate that the polarized MT network in neurons is a decisive factor in establishing axon/dendritic polarity and is initially triggered by polarized signals. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163396
Appears in Collections:气候变化与战略

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作者单位: Zhou, Z., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Xu, H., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China; Li, Y., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Yang, M., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Zhang, R., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China; Shiraishi, A., Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Biosystems Dynamics Research, Kobe, 6500047, Japan; Kiyonari, H., Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Biosystems Dynamics Research, Kobe, 6500047, Japan; Liang, X., Tsinghua-Peking Joint Center for Life Science, School of Life Sciences, Tsinghua University, Beijing, 100084, China, Max-Planck Partner Group, School of Life Sciences, Tsinghua University, Beijing, 100084, China; Huang, X., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China; Wang, Y., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Xie, Q., Chinese Academy of Chinese Medical Sciences, Beijing, 100700, China; Liu, S., Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; Chen, R., Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; Bao, L., State Key Laboratory of Cell Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China; Guo, W., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Wang, Y., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China; Meng, W., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

Recommended Citation:
Zhou Z.,Xu H.,Li Y.,et al. CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(36)
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