DOI: 10.1073/PNAS.2001768117
论文题名: ChiS is a noncanonical DNA-binding hybrid sensor kinase that directly regulates the chitin utilization program in Vibrio cholerae
作者: Klancher C.A. ; Yamamoto S. ; Dalia T.N. ; Dalia A.B.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期: 33 起始页码: 20180
结束页码: 20189
语种: 英语
英文关键词: Signal transduction
; Transcriptional regulation
; Vibrio cholerae
Scopus关键词: chitin
; protein kinase
; RNA polymerase
; sensor kinase ChiS
; unclassified drug
; bacterial protein
; chitin
; polynucleotide 5' hydroxyl kinase
; protein binding
; Article
; chromatin immunoprecipitation
; controlled study
; enzyme activation
; enzyme mechanism
; enzyme phosphorylation
; enzyme regulation
; gene expression
; nonhuman
; operon
; plasmid
; priority journal
; protein DNA binding
; protein expression
; protein function
; signal transduction
; Vibrio cholerae
; enzymology
; gene expression regulation
; genetics
; metabolism
; Vibrio cholerae
; Bacterial Proteins
; Chitin
; Gene Expression Regulation, Bacterial
; Gene Expression Regulation, Enzymologic
; Polynucleotide 5'-Hydroxyl-Kinase
; Protein Binding
; Vibrio cholerae
英文摘要: Two-component signal transduction systems (TCSs) represent a major mechanism that bacteria use to sense and respond to their environment. Prototypical TCSs are composed of a membraneembedded histidine kinase, which senses an environmental stimulus and subsequently phosphorylates a cognate partner protein called a response regulator that regulates gene expression in a phosphorylation-dependent manner. Vibrio cholerae uses the hybrid histidine kinase ChiS to activate the expression of the chitin utilization program, which is critical for the survival of this facultative pathogen in its aquatic reservoir. A cognate response regulator for ChiS has not been identified and the mechanism of ChiSdependent signal transduction remains unclear. Here, we show that ChiS is a noncanonical membrane-embedded one-component system that can both sense chitin and directly regulate gene expression via a cryptic DNA binding domain. Unlike prototypical TCSs, we find that ChiS DNA binding is diminished, rather than stimulated, by phosphorylation. Finally, we provide evidence that ChiS likely activates gene expression by directly recruiting RNA polymerase. This work addresses the mechanism of action for a major transcription factor in V. cholerae and highlights the versatility of signal transduction systems in bacterial species. © 2020 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163420
Appears in Collections: 气候变化与战略
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作者单位: Klancher, C.A., Department of Biology, Indiana University, Bloomington, IN 47405, United States; Yamamoto, S., Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan; Dalia, T.N., Department of Biology, Indiana University, Bloomington, IN 47405, United States; Dalia, A.B., Department of Biology, Indiana University, Bloomington, IN 47405, United States
Recommended Citation:
Klancher C.A.,Yamamoto S.,Dalia T.N.,et al. ChiS is a noncanonical DNA-binding hybrid sensor kinase that directly regulates the chitin utilization program in Vibrio cholerae[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(33)