DOI: 10.1073/pnas.2002008117
论文题名: On the enigmatic disappearance of Rauber’s layer
作者: van Leeuwen J. ; Rawson P. ; Berg D.K. ; Wells D.N. ; Pfeffer P.L.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期: 28 起始页码: 16409
结束页码: 16417
语种: 英语
英文关键词: Amniotic cavity
; Epiblast
; Polar trophoblast
Scopus关键词: beta galactosidase
; bone morphogenetic protein
; caspase 3
; histone H3
; protein bcl 2
; protein Nodal
; transcription factor
; transcription factor brachyury
; unclassified drug
; Wnt protein
; Brachyury protein
; fetoprotein
; protein bcl 2
; T box transcription factor
; amnion
; animal cell
; apoptosis
; Article
; bovine
; cell differentiation
; cell interaction
; cell proliferation
; controlled study
; ectoderm
; embryo
; embryo development
; gastrulation
; gene overexpression
; nonhuman
; phenotype
; placental mammal
; priority journal
; protein expression
; Rauber layer
; signal transduction
; trophoblast
; animal
; cytology
; embryology
; female
; genetics
; germ layer
; metabolism
; mouse
; pathophysiology
; trophoblast
; Animals
; Apoptosis
; Cattle
; Cell Differentiation
; Cell Proliferation
; Female
; Fetal Proteins
; Gastrulation
; Germ Layers
; Mice
; Proto-Oncogene Proteins c-bcl-2
; T-Box Domain Proteins
; Trophoblasts
英文摘要: The polar trophoblast overlays the epiblast in eutherian mammals and, depending on the species, has one of two different fates. It either remains a single-layered, thinning epithelium called “Rauber’s layer,” which soon disintegrates, or, alternatively, it keeps proliferating, contributing heavily to the population of differentiating, invasive trophoblast cells and, at least in mice, to the induction of gastrulation. While loss of the persistent polar trophoblast in mice leads to reduced induction of gastrulation, we show here that prevention of the loss of the polar trophoblast in cattle results in ectopic domains of the gastrulation marker, BRACHYURY. This phenotype, and increased epiblast proliferation, arose when Rauber’s layer was maintained for a day longer by countering apoptosis through BCL2 overexpression. This suggests that the disappearance of Rauber’s layer is a necessity, presumably to avoid excessive signaling interactions between this layer and the subjacent epiblast. We note that, in all species in which the polar trophoblast persists, including humans and mice, ectopic polar trophoblast signaling is prevented via epiblast cavitation which leads to the (pro)amniotic cavity, whose function is to distance the central epiblast from such signaling interactions. © 2020 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163432
Appears in Collections: 气候变化与战略
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作者单位: van Leeuwen, J., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand, Department of Biomedical, Molecular and Cellular Biology, Waikato University, Hamilton, 3240, New Zealand; Rawson, P., School of Biological Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand; Berg, D.K., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand; Wells, D.N., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand; Pfeffer, P.L., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand, School of Biological Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand
Recommended Citation:
van Leeuwen J.,Rawson P.,Berg D.K.,et al. On the enigmatic disappearance of Rauber’s layer[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(28)