globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1917471117
论文题名:
Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch
作者: Sánchez-Cañizares C.; Prell J.; Pini F.; Rutten P.; Kraxner K.; Wynands B.; Karunakaran R.; Poole P.S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:19
起始页码: 10234
结束页码: 10245
语种: 英语
英文关键词: Bacterial metabolism ; Nitrogen ; Plant-host interactions ; Regulatory network
Scopus关键词: exopolysaccharide ; nitrogen related phosphotransferase system ; phosphotransferase ; tricarboxylic acid ; unclassified drug ; bacterial protein ; carbon ; nitrogen ; phosphate ; phosphoenolpyruvate sugar phosphotransferase ; adaptation ; Article ; bacterial metabolism ; bacterial phenomena and functions ; bacterial strain ; carbon metabolism ; cell survival ; controlled study ; enzyme activity ; gene regulatory network ; homeostasis ; nitrogen metabolism ; nitrogen uptake ; nonhuman ; potassium transport ; priority journal ; protein interaction ; protein modification ; protein phosphorylation ; Proteobacteria ; Rhizobium leguminosarum ; signal transduction ; citric acid cycle ; gene expression regulation ; genetics ; growth, development and aging ; metabolism ; phosphorylation ; promoter region ; Bacterial Proteins ; Carbon ; Citric Acid Cycle ; Gene Expression Regulation, Bacterial ; Nitrogen ; Phosphates ; Phosphoenolpyruvate Sugar Phosphotransferase System ; Phosphorylation ; Promoter Regions, Genetic ; Rhizobium leguminosarum
英文摘要: The nitrogen-related phosphotransferase system (PTSNtr) of Rhizobium leguminosarum bv. viciae 3841 transfers phosphate from PEP via PtsP and NPr to two output regulators, ManX and PtsN. ManX controls central carbon metabolism via the tricarboxylic acid (TCA) cycle, while PtsN controls nitrogen uptake, exopolysaccharide production, and potassium homeostasis, each of which is critical for cellular adaptation and survival. Cellular nitrogen status modulates phosphorylation when glutamine, an abundant amino acid when nitrogen is available, binds to the GAF sensory domain of PtsP, preventing PtsP phosphorylation and subsequent modification of ManX and PtsN. Under nitrogen-rich, carbon-limiting conditions, unphosphorylated ManX stimulates the TCA cycle and carbon oxidation, while unphosphorylated PtsN stimulates potassium uptake. The effects are reversed with the phosphorylation of ManX and PtsN, occurring under nitrogen-limiting, carbon-rich conditions; phosphorylated PtsN triggers uptake and nitrogen metabolism, the TCA cycle and carbon oxidation are decreased, while carbon-storage polymers such as surface polysaccharide are increased. Deleting the GAF domain from PtsP makes cells "blind" to the cellular nitrogen status. PTSNtr constitutes a switch through which carbon and nitrogen metabolism are rapidly, and reversibly, regulated by protein:protein interactions. PTSNtr is widely conserved in proteobacteria, highlighting its global importance. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163467
Appears in Collections:气候变化与战略

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作者单位: Sánchez-Cañizares, C., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom; Prell, J., Soil Ecology, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, D-52056, Germany; Pini, F., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom; Rutten, P., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom; Kraxner, K., Soil Ecology, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, D-52056, Germany; Wynands, B., Soil Ecology, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, D-52056, Germany; Karunakaran, R., Department of Molecular Microbiology, John Innes Centre, Norwich, NR4 7UH, United Kingdom; Poole, P.S., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom

Recommended Citation:
Sánchez-Cañizares C.,Prell J.,Pini F.,et al. Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(19)
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