globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1920143117
论文题名:
The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus
作者: Medico L.D.; Cerletti D.; Schächle P.; Christen M.; Christen B.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:17
起始页码: 9546
结束页码: 9553
语种: 英语
英文关键词: C-di-GMP ; Caulobacter crescentus ; FRET microscopy ; TnSeq ; Type IV pilin
Scopus关键词: bacterial protein ; cyclic GMP ; monomer ; periplasmic protein ; pilin ; PleC kinase ; unclassified drug ; fimbria protein ; Article ; bacterial colonization ; Caulobacter vibrioides ; cell cycle checkpoint ; controlled study ; fluorescence resonance energy transfer ; gene cluster ; gene expression regulation ; gene identification ; gene location ; gene sequence ; genetic susceptibility ; inner membrane ; membrane potential ; molecular dynamics ; nonhuman ; periplasm ; PilA gene ; priority journal ; protein synthesis ; signal transduction ; transposon ; bacterium adherence ; Caulobacter vibrioides ; cell cycle ; genetics ; metabolism ; physiology ; Bacterial Adhesion ; Caulobacter crescentus ; Cell Cycle ; Fimbriae Proteins ; Gene Expression Regulation, Bacterial
英文摘要: Understanding how bacteria colonize surfaces and regulate cell-cycle progression in response to cellular adhesion is of fundamental importance. Here, we use transposon sequencing in conjunction with fluorescence resonance energy transfer (FRET) microscopy to uncover the molecular mechanism for how surface sensing drives cell-cycle initiation in Caulobacter crescentus. We identify the type IV pilin protein PilA as the primary signaling input that couples surface contact to cell-cycle initiation via the second messenger cyclic di-GMP (c-di-GMP). Upon retraction of pili filaments, the monomeric pilin reservoir in the inner membrane is sensed by the 17-amino acid transmembrane helix of PilA to activate the PleC-PleD two-component signaling system, increase cellular c-di-GMP levels, and signal the onset of the cell cycle. We termed the PilA signaling sequence CIP for “cell-cycle initiating pilin” peptide. Addition of the chemically synthesized CIP peptide initiates cell-cycle progression and simultaneously inhibits surface attachment. The broad conservation of the type IV pili and their importance in pathogens for host colonization suggests that CIP peptide mimetics offer strategies to inhibit surface sensing, prevent biofilm formation and control persistent infections. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163471
Appears in Collections:气候变化与战略

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作者单位: Medico, L.D., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Cerletti, D., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Schächle, P., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Christen, M., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Christen, B., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland

Recommended Citation:
Medico L.D.,Cerletti D.,Schächle P.,et al. The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(17)
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