globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1909498116
论文题名:
Intact carbonic acid is a viable protonating agent for biological bases
作者: Aminov D.; Pines D.; Kiefer P.M.; Daschakraborty S.; Hynes J.T.; Pines E.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:42
起始页码: 20837
结束页码: 20843
语种: 英语
英文关键词: Biological ; Blood plasma ; Carbonic acid ; Protonation
Scopus关键词: base ; carbon dioxide ; carbonic acid ; carboxylic acid ; lactic acid ; proton ; water ; Article ; human ; kinetics ; membrane ; molecular dynamics ; nonhuman ; plasma ; priority journal ; protonation ; temperature dependence ; ultraviolet visible spectroscopy
英文摘要: Carbonic acid H2CO3 (CA) is a key constituent of the universal CA/ bicarbonate/CO2 buffer maintaining the pH of both blood and the oceans. Here we demonstrate the ability of intact CA to quantitatively protonate bases with biologically-relevant pKas and argue that CA has a previously unappreciated function as a major source of protons in blood plasma. We determine with high precision the temperature dependence of pKa(CA), pKa(T) = −373.604 + 16,500/T + 56.478 ln T. At physiological-like conditions pKa(CA) = 3.45 (I = 0.15 M, 37 °C), making CA stronger than lactic acid. We further demonstrate experimentally that CA decomposition to H2O and CO2 does not impair its ability to act as an ordinary carboxylic acid and to efficiently protonate physiological-like bases. The consequences of this conclusion are far reaching for human physiology and marine biology. While CA is somewhat less reactive than (H+)aq, it is more than 1 order of magnitude more abundant than (H+)aq in the blood plasma and in the oceans. In particular, CA is about 70× more abundant than (H+)aq in the blood plasma, where we argue that its overall protonation efficiency is 10 to 20× greater than that of (H+)aq, often considered to be the major protonating agent there. CA should thus function as a major source for fast in vivo acid–base reactivity in the blood plasma, possibly penetrating intact into membranes and significantly helping to compensate for (H+)aq’s kinetic deficiency in sustaining the large proton fluxes that are vital for metabolic processes and rapid enzymatic reactions. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163522
Appears in Collections:气候变化与战略

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作者单位: Aminov, D., Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel; Pines, D., Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel; Kiefer, P.M., Department of Chemistry, University of Colorado Boulder, Boulder, CO 80309-0215, United States; Daschakraborty, S., Department of Chemistry, University of Colorado Boulder, Boulder, CO 80309-0215, United States, Department of Chemistry, Indian Institute of Technology (IIT) Patna, Bihta, Patna, 801106, India; Hynes, J.T., Department of Chemistry, University of Colorado Boulder, Boulder, CO 80309-0215, United States, PASTEUR, Départmente de Chimie, Ecole Normale Supérieure, PSL Research University, Sorbonne Université, UPMC Université Paris 06, CNRS, Paris, 75005, France; Pines, E., Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel

Recommended Citation:
Aminov D.,Pines D.,Kiefer P.M.,et al. Intact carbonic acid is a viable protonating agent for biological bases[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(42)
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