globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1821038116
论文题名:
Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties
作者: Boeynaems S.; Holehouse A.S.; Weinhardt V.; Kovacs D.; Van Lindt J.; Larabell C.; Bosch L.V.D.; Das R.; Tompa P.S.; Pappu R.V.; Gitler A.D.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:16
起始页码: 7889
结束页码: 7898
语种: 英语
英文关键词: Biomolecular condensates ; Complex coacervation ; Intrinsically disordered proteins ; Phase transitions ; RNA
Scopus关键词: archetypal RNA ; peptide derivative ; RNA ; unclassified drug ; intrinsically disordered protein ; RNA ; Article ; experimental study ; molecular dynamics ; molecular genetics ; molecular model ; priority journal ; protein RNA binding ; RNA sequence ; RNA structure ; simulation ; biology ; cell organelle ; chemistry ; metabolism ; molecular dynamics ; phase transition ; physiology ; Computational Biology ; Intrinsically Disordered Proteins ; Molecular Dynamics Simulation ; Organelles ; Phase Transition ; RNA
英文摘要: Phase separation of multivalent protein and RNA molecules underlies the biogenesis of biomolecular condensates such as membraneless organelles. In vivo, these condensates encompass hundreds of distinct types of molecules that typically organize into multilayered structures supporting the differential partitioning of molecules into distinct regions with distinct material properties. The interplay between driven (active) versus spontaneous (passive) processes that are required for enabling the formation of condensates with coexisting layers of distinct material properties remains unclear. Here, we deploy systematic experiments and simulations based on coarse-grained models to show that the collective interactions among the simplest, biologically relevant proteins and archetypal RNA molecules are sufficient for driving the spontaneous emergence of multilayered condensates with distinct material properties. These studies yield a set of rules regarding homotypic and heterotypic interactions that are likely to be relevant for understanding the interplay between active and passive processes that control the formation of functional biomolecular condensates. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163561
Appears in Collections:气候变化与战略

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作者单位: Boeynaems, S., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Holehouse, A.S., Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, United States, Center for Science and Engineering of Living Systems, Washington University, St. Louis, MO 63130, United States; Weinhardt, V., Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States, Department of Anatomy, University of California, San Francisco, CA 94143, United States; Kovacs, D., Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, B-1050, Belgium; Van Lindt, J., Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, B-1050, Belgium; Larabell, C., Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States, Department of Anatomy, University of California, San Francisco, CA 94143, United States; Bosch, L.V.D., Laboratory of Neurobiology, Center for Brain and Disease Research, Vlaams Instituut voor Biotechnologie, Leuven, 3000, Belgium, Experimental Neurology, Department of Neurosciences, KU Leuven, Leuven, 3001, Belgium; Das, R., Department of Biochemistry, Stanford University, Stanford, CA 94305, United States, Department of Physics, Stanford University, Stanford, CA 94305, United States; Tompa, P.S., Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, B-1050, Belgium, Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, H-1117, Hungary; Pappu, R.V., Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, United States, Center for Science and Engineering of Living Systems, Washington University, St. Louis, MO 63130, United States; Gitler, A.D., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States

Recommended Citation:
Boeynaems S.,Holehouse A.S.,Weinhardt V.,et al. Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(16)
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