globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1904602116
论文题名:
The constrained architecture of mammalian Hox gene clusters
作者: Darbellay F.; Bochaton C.; Lopez-Delisle L.; Mascrez B.; Tschopp P.; Delpretti S.; Zakany J.; Duboule D.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:27
起始页码: 13424
结束页码: 13433
语种: 英语
英文关键词: CRISPR-Cas9 ; CTCF ; Hox ; Polycomb ; Transcription
Scopus关键词: transcription factor CTCF ; adult ; allele ; animal tissue ; Article ; controlled study ; embryo ; fetus ; gene cluster ; gene control ; gene locus ; genetic engineering ; genetic transcription ; Hox gene ; mammal ; mouse ; nonhuman ; priority journal
英文摘要: In many animal species with a bilateral symmetry, Hox genes are clustered either at one or at several genomic loci. This organization has a functional relevance, as the transcriptional control applied to each gene depends upon its relative position within the gene cluster. It was previously noted that vertebrate Hox clusters display a much higher level of genomic organization than their invertebrate counterparts. The former are always more compact than the latter, they are generally devoid of repeats and of interspersed genes, and all genes are transcribed by the same DNA strand, suggesting that particular factors constrained these clusters toward a tighter structure during the evolution of the vertebrate lineage. Here, we investigate the importance of uniform transcriptional orientation by engineering several alleles within the HoxD cluster, such as to invert one or several transcription units, with or without a neighboring CTCF site. We observe that the association between the tight structure of mammalian Hox clusters and their regulation makes inversions likely detrimental to the proper implementation of this complex genetic system. We propose that the consolidation of Hox clusters in vertebrates, including transcriptional polarity, evolved in conjunction with the emergence of global gene regulation via the flanking regulatory landscapes, to optimize a coordinated response of selected subsets of target genes in cis. © 2019 National Academy of Sciences. All rights reserved.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163604
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Darbellay, F., School of Life Sciences, Federal Institute of Technology, Lausanne, Lausanne, 1015, Switzerland; Bochaton, C., School of Life Sciences, Federal Institute of Technology, Lausanne, Lausanne, 1015, Switzerland; Lopez-Delisle, L., School of Life Sciences, Federal Institute of Technology, Lausanne, Lausanne, 1015, Switzerland; Mascrez, B., Department of Genetics and Evolution, University of Geneva, Geneva 4, 1211, Switzerland; Tschopp, P., Department of Genetics and Evolution, University of Geneva, Geneva 4, 1211, Switzerland, Department of Environmental Sciences Zoology, University of Basel, Basel, 4051, Switzerland; Delpretti, S., School of Life Sciences, Federal Institute of Technology, Lausanne, Lausanne, 1015, Switzerland, AC Immune S.A., Lausanne, 1015, Switzerland; Zakany, J., Department of Genetics and Evolution, University of Geneva, Geneva 4, 1211, Switzerland; Duboule, D., School of Life Sciences, Federal Institute of Technology, Lausanne, Lausanne, 1015, Switzerland, Department of Genetics and Evolution, University of Geneva, Geneva 4, 1211, Switzerland, Collège de France, Paris, 75005, France

Recommended Citation:
Darbellay F.,Bochaton C.,Lopez-Delisle L.,et al. The constrained architecture of mammalian Hox gene clusters[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(27)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Darbellay F.]'s Articles
[Bochaton C.]'s Articles
[Lopez-Delisle L.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Darbellay F.]'s Articles
[Bochaton C.]'s Articles
[Lopez-Delisle L.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Darbellay F.]‘s Articles
[Bochaton C.]‘s Articles
[Lopez-Delisle L.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.