DOI: 10.1073/pnas.1811931115
论文题名: Baseplate variability of Vibrio cholerae chemoreceptor arrays
作者: Yang W. ; Alvarado A. ; Glatter T. ; Ringgaard S. ; Briegel A.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 52 起始页码: 13365
结束页码: 13370
语种: 英语
英文关键词: Bacterial chemotaxis
; Baseplate
; Chemoreceptor array
; Electron cryotomography
; Vibrio
Scopus关键词: bacterial protein
; CheA kinase
; CheV protein
; ParP protein
; unclassified drug
; bacterial protein
; Escherichia coli protein
; membrane protein
; methyl accepting chemotaxis protein
; protein binding
; Article
; bacterial flagellum
; cellular distribution
; chemoreceptor
; controlled study
; Escherichia coli
; kinetosome
; nonhuman
; operon
; priority journal
; stoichiometry
; Vibrio cholerae
; archaeon
; bacterium
; chemoreceptor cell
; chemotaxis
; genetic variation
; genetics
; metabolism
; physiology
; signal transduction
; Vibrio cholerae
; Archaea
; Bacteria
; Bacterial Proteins
; Chemoreceptor Cells
; Chemotaxis
; Escherichia coli
; Escherichia coli Proteins
; Genetic Variation
; Membrane Proteins
; Methyl-Accepting Chemotaxis Proteins
; Protein Binding
; Signal Transduction
; Vibrio cholerae
英文摘要: The chemoreceptor array, a remarkably ordered supramolecular complex, is composed of hexagonally packed trimers of receptor dimers networked by a histidine kinase and one or more coupling proteins. Even though the receptor packing is universal among chemotactic bacteria and archaea, the array architecture has been extensively studied only in selected model organisms. Here, we show that even in the complete absence of the kinase, the cluster II arrays in Vibrio cholerae retain their native spatial localization and the iconic hexagonal packing of the receptors with 12-nm spacing. Our results demonstrate that the chemotaxis array is versatile in composition, a property that allows auxiliary chemotaxis proteins such as ParP and CheV to integrate directly into the assembly. Along with its compositional variability, cluster II arrays exhibit a low degree of structural stability compared with the ultrastable arrays in Escherichia coli. We propose that the variability in chemoreceptor arrays is an important mechanism that enables the incorporation of chemotaxis proteins based on their availability. © 2018 National Academy of Sciences. All Rights Reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163625
Appears in Collections: 气候变化与战略
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作者单位: Yang, W., Institute of Biology, Leiden University, Leiden, 2333 BE, Netherlands; Alvarado, A., Department of Ecophysiology, Max Plank Institute for Terrestrial Microbiology, Marburg, 35043, Germany; Glatter, T., Core Facility for Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Marburg, 35043, Germany; Ringgaard, S., Department of Ecophysiology, Max Plank Institute for Terrestrial Microbiology, Marburg, 35043, Germany; Briegel, A., Institute of Biology, Leiden University, Leiden, 2333 BE, Netherlands
Recommended Citation:
Yang W.,Alvarado A.,Glatter T.,et al. Baseplate variability of Vibrio cholerae chemoreceptor arrays[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(52)