DOI: 10.1073/pnas.1814048115
论文题名: Calditol-linked membrane lipids are required for acid tolerance in Sulfolobus acidocaldarius
作者: Zeng Z. ; Liu X.-L. ; Wei J.H. ; Summons R.E. ; Welander P.V.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 51 起始页码: 12932
结束页码: 12937
语种: 英语
英文关键词: Calditol
; GDGT
; Glycerol dibiphytany
; Glycerol tetraethers
; Radical SAM
; Sulfolobus
Scopus关键词: calditol synthase
; glycerol dibiphytanyl glycerol tetraether
; membrane lipid
; s adenosylmethionine
; synthetase
; unclassified drug
; archaeal protein
; membrane lipid
; acid tolerance
; archaeal cell
; Article
; controlled study
; derivatization
; enzyme mechanism
; metagenome
; nonhuman
; pH
; phylogeny
; priority journal
; protein function
; protein synthesis
; salinity
; Sulfolobus acidocaldarius
; archaeal genome
; chemistry
; genetics
; metabolism
; physiological stress
; physiology
; Sulfolobus acidocaldarius
; Archaeal Proteins
; Genome, Archaeal
; Hydrogen-Ion Concentration
; Membrane Lipids
; Stress, Physiological
; Sulfolobus acidocaldarius
英文摘要: Archaea have many unique physiological features of which the lipid composition of their cellular membranes is the most striking. Archaeal ether-linked isoprenoidal membranes can occur as bilayers or monolayers, possess diverse polar head groups, and a multiplicity of ring structures in the isoprenoidal cores. These lipid structures are proposed to provide protection from the extreme temperature, pH, salinity, and nutrient-starved conditions that many archaea inhabit. However, many questions remain regarding the synthesis and physiological role of some of the more complex archaeal lipids. In this study, we identify a radical S-adenosylmethionine (SAM) protein in Sulfolobus acidocaldarius required for the synthesis of a unique cyclopentyl head group, known as calditol. Calditol-linked glycerol dibiphytanyl glycerol tetraethers (GDGTs) are membrane spanning lipids in which calditol is ether bonded to the glycerol backbone and whose production is restricted to a subset of thermoacidophilic archaea of the Sulfolobales order within the Crenarchaeota phylum. Several studies have focused on the enzymatic mechanism for the synthesis of the calditol moiety, but to date no protein that catalyzes this reaction has been discovered. Phylogenetic analyses of this putative calditol synthase (Cds) reveal the genetic potential for calditol–GDGT synthesis in phyla other than the Crenarchaeota, including the Korarchaeota and Marsarchaeota. In addition, we identify Cds homologs in metagenomes predominantly from acidic ecosystems. Finally, we demonstrate that deletion of calditol synthesis renders S. acidocaldarius sensitive to extremely low pH, indicating that calditol plays a critical role in protecting archaeal cells from acidic stress. © 2018 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163628
Appears in Collections: 气候变化与战略
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作者单位: Zeng, Z., Department of Earth System Science, Stanford University, Stanford, CA 94305, United States; Liu, X.-L., Department of Geology and Geophysics, University of Oklahoma, Norman, OK 73019, United States; Wei, J.H., Department of Earth System Science, Stanford University, Stanford, CA 94305, United States; Summons, R.E., Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Welander, P.V., Department of Earth System Science, Stanford University, Stanford, CA 94305, United States
Recommended Citation:
Zeng Z.,Liu X.-L.,Wei J.H.,et al. Calditol-linked membrane lipids are required for acid tolerance in Sulfolobus acidocaldarius[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(51)