globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1813058115
论文题名:
Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity
作者: Wang Q.; Li Y.; Ishikawa K.; Kosami K.-I.; Uno K.; Nagawa S.; Tan L.; Du J.; Shimamoto K.; Kawano Y.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:49
起始页码: E11551
结束页码: E11560
语种: 英语
英文关键词: Effector-triggered immunity ; GEF ; R protein ; Rice ; Small GTPase
Scopus关键词: guanine nucleotide exchange factor ; Pit protein ; plant protein ; Rac1 protein ; SPK1 protein ; unclassified drug ; plant protein ; Article ; cell death ; controlled study ; in vitro study ; in vivo study ; Magnaporthe oryzae ; nonhuman ; Pia gene ; Pit gene ; plant gene ; plant genome ; plant immunity ; priority journal ; protein analysis ; protein binding ; protein domain ; protein function ; protein interaction ; rice ; signal transduction ; gene expression regulation ; genetics ; immunology ; Magnaporthe ; metabolism ; microbiology ; Oryza ; plant disease ; Gene Expression Regulation, Plant ; Magnaporthe ; Oryza ; Plant Diseases ; Plant Proteins
英文摘要: Resistance (R) genes encode intracellular nucleotide-binding/ leucine-rich repeat-containing (NLR) family proteins that serve as critical plant immune receptors to induce effector-triggered immunity (ETI). NLR proteins possess a tripartite domain architecture consisting of an N-terminal variable region, a central nucleotide-binding domain, and a C-terminal leucine-rich repeat. N-terminal coiled-coil (CC) or Toll-interleukin 1 receptor (TIR) domains of R proteins appear to serve as platforms to trigger immune responses, because overexpression of the CC or TIR domain of some R proteins is sufficient to induce an immune response. Because direct downstream signaling molecules of R proteins remain obscure, the molecular mechanisms by which R proteins regulate downstream signaling are largely unknown. We reported previously that a rice R protein named Pit triggers ETI through a small GTPase, OsRac1, although how Pit activates OsRac1 is unclear. Here, we identified OsSPK1, a DOCK family guanine nucleotide exchange factor, as an interactor of Pit and activator for OsRac1. OsSPK1 contributes to signaling by two disease-resistance genes, Pit and Pia, against the rice blast fungus Magnaporthe oryzae and facilitates OsRac1 activation in vitro and in vivo. The CC domain of Pit is required for its binding to OsSPK1, OsRac1 activation, and the induction of cell death. Overall, we conclude that OsSPK1 is a direct and key signaling target of Pit-mediated immunity. Our results shed light on how R proteins trigger ETI through direct downstream molecules. © 2018 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163636
Appears in Collections:气候变化与战略

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作者单位: Wang, Q., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Li, Y., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, University of Chinese Academy of Sciences, Beijing, 100049, China; Ishikawa, K., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, Department of Genomics and Breeding, Iwate Biotechnology Research Center, Iwate, 024-0003, Japan; Kosami, K.-I., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China; Uno, K., Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Ikoma Nara, 630-0101, Japan; Nagawa, S., Core Facility of Cell Biology, Shanghai Center for Plant Stress Biology, Shanghai, 201602, China, Fujian Agriculture and Forestry University, University of California, Riverside Joint Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou Fujian, 350002, China, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou Fujian, 350002, China; Tan, L., Core Facility of Cell Biology, Shanghai Center for Plant Stress Biology, Shanghai, 201602, China; Du, J., National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Shanghai, 201602, China; Shimamoto, K., Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Ikoma Nara, 630-0101, Japan; Kawano, Y., Signal Transduction and Immunity Group, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China, Kihara Institute for Biological Research, Yokohama City University, Kanagawa, 244-0813, Japan

Recommended Citation:
Wang Q.,Li Y.,Ishikawa K.,et al. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(49)
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