globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1810706115
论文题名:
Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability
作者: Moss F.R.; Shuken S.R.; Mercer J.A.M.; Cohen C.M.; Weiss T.M.; Boxer S.G.; Burns N.Z.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:37
起始页码: 9098
结束页码: 9103
语种: 英语
英文关键词: Anammox ; Ladderane ; Lipid bilayer ; Membrane structure ; Proton permeability
Scopus关键词: hydrazine ; hydroxide ; Ladderane phospholipid ; phosphatidylcholine ; phospholipid derivative ; proton ; unclassified drug ; hydrazine derivative ; hydroxide ; phospholipid ; anammox bacterium ; Article ; bioassay ; bleaching ; controlled study ; fluorescence ; lipid analysis ; lipid bilayer ; membrane permeability ; nonhuman ; pH ; priority journal ; solubility ; synthesis ; anaerobic growth ; bacterium ; cell membrane ; cell membrane permeability ; chemistry ; metabolism ; physiology ; Anaerobiosis ; Bacteria ; Cell Membrane ; Cell Membrane Permeability ; Hydrazines ; Hydroxides ; Phospholipids ; Protons
英文摘要: Ladderane lipids are unique to anaerobic ammonium-oxidizing (anammox) bacteria and are enriched in the membrane of the anammoxosome, an organelle thought to compartmentalize the anammox process, which involves the toxic intermediate hydrazine (N2H4). Due to the slow growth rate of anammox bacteria and difficulty of isolating pure ladderane lipids, experimental evidence of the biological function of ladderanes is lacking. We have synthesized two natural and one unnatural ladderane phosphatidylcholine lipids and compared their thermotropic properties in self-assembled bilayers to distinguish between [3]- and [5]-ladderane function. We developed a hydrazine transmembrane diffusion assay using a water-soluble derivative of a hydrazine sensor and determined that ladderane membranes are as permeable to hydrazine as straight-chain lipid bilayers. However, pH equilibration across ladderane membranes occurs 5–10 times more slowly than across straight-chain lipid membranes. Langmuir monolayer analysis and the rates of fluorescence recovery after photobleaching suggest that dense ladderane packing may preclude formation of proton/hydroxide-conducting water wires. These data support the hypothesis that ladderanes prevent the breakdown of the proton motive force rather than blocking hydrazine transmembrane diffusion in anammox bacteria. © 2018 National Academy of Sciences. All Rights Reserved.
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被引频次[WOS]:43   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163661
Appears in Collections:气候变化与战略

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作者单位: Moss, F.R., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Shuken, S.R., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Mercer, J.A.M., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Cohen, C.M., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Weiss, T.M., Stanford Synchrotron Radiation Laboratory, Stanford Linear Accelerator Center, Stanford University, Menlo Park, CA 94025, United States; Boxer, S.G., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Burns, N.Z., Department of Chemistry, Stanford University, Stanford, CA 94305, United States

Recommended Citation:
Moss F.R.,Shuken S.R.,Mercer J.A.M.,et al. Ladderane phospholipids form a densely packed membrane with normal hydrazine and anomalously low proton/hydroxide permeability[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(37)
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