globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1722335115
论文题名:
MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health
作者: Stringlis I.A.; Yu K.; Feussner K.; De Jonge R.; Van Bentum S.; Van Verk M.C.; Berendsen R.L.; Bakker P.A.H.M.; Feussner I.; Pieterse C.M.J.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:22
起始页码: E5213
结束页码: E5222
语种: 英语
英文关键词: Coumarin ; Induced systemic resistance ; Iron-deficiency response ; Microbiome assembly ; Root metabolome
Scopus关键词: beta glucosidase ; coumarin ; glucoside ; protein BGLU42 ; protein MYB72 ; scopoletin ; scopolin ; transcription factor ; unclassified drug ; Arabidopsis protein ; iron ; Myb72 protein, Arabidopsis ; scopoletin ; transcription factor ; Arabidopsis thaliana ; Article ; bacterial microbiome ; controlled study ; enzyme activity ; Fusarium oxysporum ; gene sequence ; metagenome ; microbial community ; microbiome assembly ; mycobiome ; nonhuman ; organismal interaction ; plant health ; plant immunity ; plant physiology ; plant root ; priority journal ; protein function ; protein synthesis ; Pseudomonas ; Pseudomonas capeferrum ; Pseudomonas simiae ; rhizosphere ; Verticillium dahliae ; Arabidopsis ; Fusarium ; metabolism ; metabolome ; microbiology ; microflora ; physiology ; Verticillium ; Arabidopsis ; Arabidopsis Proteins ; Fusarium ; Iron ; Metabolome ; Microbiota ; Plant Roots ; Pseudomonas ; Rhizosphere ; Scopoletin ; Transcription Factors ; Verticillium
英文摘要: Plant roots nurture a tremendous diversity of microbes via exudation of photosynthetically fixed carbon sources. In turn, probiotic members of the root microbiome promote plant growth and protect the host plant against pathogens and pests. In the Arabidopsis thaliana–Pseudomonas simiae WCS417 model system the root-specific transcription factor MYB72 and the MYB72-controlled β-glucosidase BGLU42 emerged as important regulators of beneficial rhizobacteria-induced systemic resistance (ISR) and iron-uptake responses. MYB72 regulates the biosynthesis of iron-mobilizing fluorescent phenolic compounds, after which BGLU42 activity is required for their excretion into the rhizosphere. Metabolite fingerprinting revealed the antimicrobial coumarin scopoletin as a dominant metabolite that is produced in the roots and excreted into the rhizosphere in a MYB72- and BGLU42-dependent manner. Shotgun-metagenome sequencing of root-associated microbiota of Col-0, myb72, and the scopoletin biosynthesis mutant f6′h1 showed that scopoletin selectively impacts the assembly of the microbial community in the rhizosphere. We show that scopoletin selectively inhibits the soil-borne fungal pathogens Fusarium oxysporum and Verticillium dahliae, while the growth-promoting and ISR-inducing rhizobacteria P. simiae WCS417 and Pseudomonas capeferrum WCS358 are highly tolerant of the antimicrobial effect of scopoletin. Collectively, our results demonstrate a role for coumarins in microbiome assembly and point to a scenario in which plants and probiotic rhizobacteria join forces to trigger MYB72/BGLU42-dependent scopolin production and scopoletin excretion, resulting in improved niche establishment for the microbial partner and growth and immunity benefits for the host plant. © 2018 National Academy of Sciences. All rights reserved.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163706
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Stringlis, I.A., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Yu, K., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Feussner, K., Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Göttingen, Göttingen, 37077, Germany; De Jonge, R., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands, Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, Ghent, 9052, Belgium, Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9052, Belgium; Van Bentum, S., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Van Verk, M.C., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Berendsen, R.L., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Bakker, P.A.H.M., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Feussner, I., Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Göttingen, Göttingen, 37077, Germany, Department of Plant Biochemistry, Göttingen Center for Molecular Biosciences, University of Göttingen, Göttingen, 37077, Germany; Pieterse, C.M.J., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands

Recommended Citation:
Stringlis I.A.,Yu K.,Feussner K.,et al. MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(22)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Stringlis I.A.]'s Articles
[Yu K.]'s Articles
[Feussner K.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Stringlis I.A.]'s Articles
[Yu K.]'s Articles
[Feussner K.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Stringlis I.A.]‘s Articles
[Yu K.]‘s Articles
[Feussner K.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.