globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1800635115
论文题名:
Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein
作者: Mahatabuddin S.; Fukami D.; Arai T.; Nishimiya Y.; Shimizu R.; Shibazaki C.; Kondo H.; Adachi M.; Tsuda S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:21
起始页码: 5456
结束页码: 5461
语种: 英语
英文关键词: Crystal structure ; Hydration shell ; Ice-binding protein ; Polypentagonal waters ; Water cluster
Scopus关键词: antifreeze protein ; ice binding protein ; unclassified drug ; antifreeze protein ; carrier protein ; fish protein ; ice ; isoprotein ; protein binding ; water ; Article ; controlled study ; enzyme activity ; enzyme inhibition ; hydrogen bond ; information processing ; Macrozoarces americanus ; nonhuman ; Perciformes ; polymerase chain reaction ; polypentagonal ice like water network ; priority journal ; protein expression ; protein function ; animal ; binding site ; biophysics ; chemistry ; fish ; freezing ; genetics ; metabolism ; mutation ; protein conformation ; X ray crystallography ; Animals ; Antifreeze Proteins ; Binding Sites ; Biophysical Phenomena ; Carrier Proteins ; Crystallography, X-Ray ; Fish Proteins ; Fishes ; Freezing ; Ice ; Mutation ; Protein Binding ; Protein Conformation ; Protein Isoforms ; Water
英文摘要: Polypentagonal water networks were recently observed in a protein capable of binding to ice crystals, or ice-binding protein (IBP). To examine such water networks and clarify their role in ice-binding, we determined X-ray crystal structures of a 65-residue defective isoform of a Zoarcidae-derived IBP (wild type, WT) and its five single mutants (A20L, A20G, A20T, A20V, and A20I). Polypentagonal water networks composed of ∼50 semiclathrate waters were observed solely on the strongest A20I mutant, which appeared to include a tetrahedral water cluster exhibiting a perfect position match to the (1010) first prism plane of a single ice crystal. Inclusion of another symmetrical water cluster in the polypentagonal network showed a perfect complementarity to the waters constructing the (2021) pyramidal ice plane. The order of ice-binding strength was A20L < A20G < WT < A20T < A20V < A20I, where the top three mutants capable of binding to the first prism and the pyramidal ice planes commonly contained a bifurcated γ-CH3 group. These results suggest that a fine-tuning of the surface of Zoarcidae-derived IBP assisted by a side-chain group regulates the holding property of its polypentagonal water network, the function of which is to freeze the host protein to specific ice planes. © 2018 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163711
Appears in Collections:气候变化与战略

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作者单位: Mahatabuddin, S., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Fukami, D., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Arai, T., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Nishimiya, Y., Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan; Shimizu, R., Quantum Beam Science Research Directorate, National Institute for Quantum and Radiological Science and Technology (QST), Ibaraki, 319-1106, Japan; Shibazaki, C., Quantum Beam Science Research Directorate, National Institute for Quantum and Radiological Science and Technology (QST), Ibaraki, 319-1106, Japan; Kondo, H., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan; Adachi, M., Quantum Beam Science Research Directorate, National Institute for Quantum and Radiological Science and Technology (QST), Ibaraki, 319-1106, Japan; Tsuda, S., Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan, Operando Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology, Tsukuba, 305-8563, Japan

Recommended Citation:
Mahatabuddin S.,Fukami D.,Arai T.,et al. Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(21)
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