globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1719659115
论文题名:
Abscisic acid-induced degradation of Arabidopsis guanine nucleotide exchange factor requires calcium-dependent protein kinases
作者: Li Z.; Takahashi Y.; Scavo A.; Brandt B.; Nguyen D.; Rieu P.; Schroeder J.I.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:19
起始页码: E4522
结束页码: E4531
语种: 英语
英文关键词: ABA ; Abiotic stress ; CPK ; Protein phosphorylation and degradation ; RopGEF
Scopus关键词: abscisic acid ; calcium dependent protein kinase ; guanine nucleotide exchange factor ; protein cpk11 ; protein cpk3 ; protein cpk4 ; protein cpk6 ; protein kinase ; protein RopGEF1 ; unclassified drug ; abscisic acid ; Arabidopsis protein ; calcium calmodulin dependent protein kinase ; CPK4 protein, Arabidopsis ; guanine nucleotide exchange factor ; phytohormone ; RopGEF1 protein, Arabidopsis ; amino terminal sequence ; Arabidopsis ; Article ; controlled study ; in vivo study ; nonhuman ; phenotype ; priority journal ; protein degradation ; protein function ; protein interaction ; protein phosphorylation ; protein protein interaction ; root development ; root hair ; drug effect ; gene expression regulation ; genetics ; growth, development and aging ; metabolism ; phosphorylation ; plant root ; protein degradation ; protein transport ; signal transduction ; Abscisic Acid ; Arabidopsis ; Arabidopsis Proteins ; Calcium-Calmodulin-Dependent Protein Kinases ; Gene Expression Regulation, Plant ; Guanine Nucleotide Exchange Factors ; Phosphorylation ; Plant Growth Regulators ; Plant Roots ; Protein Transport ; Proteolysis ; Signal Transduction
英文摘要: Abscisic acid (ABA) plays essential roles in plant development and responses to environmental stress. ABA induces subcellular translocation and degradation of the guanine nucleotide exchange factor RopGEF1, thus facilitating ABA core signal transduction. However, the underlying mechanisms for ABA-triggered RopGEF1 trafficking/degradation remain unknown. Studies have revealed that RopGEFs associate with receptor-like kinases to convey developmental signals to small ROP GTPases. However, how the activities of RopGEFs are modulated is not well understood. Type 2C protein phosphatases stabilize the RopGEF1 protein, indicating that phosphorylation may trigger RopGEF1 trafficking and degradation. We have screened inhibitors followed by several protein kinase mutants and find that quadruple-mutant plants in the Arabidopsis calcium-dependent protein kinases (CPKs) cpk3/4/6/11 disrupt ABA-induced trafficking and degradation of RopGEF1. Moreover, cpk3/4/6/11 partially impairs ABA inhibition of cotyledon emergence. Several CPKs interact with RopGEF1. CPK4 binds to and phosphorylates RopGEF1 and promotes the degradation of RopGEF1. CPK-mediated phosphorylation of RopGEF1 at specific N-terminal serine residues causes the degradation of RopGEF1 and mutation of these sites also compromises the RopGEF1 overexpression phenotype in root hair development in Arabidopsis. Our findings establish the physiological and molecular functions and relevance of CPKs in regulation of RopGEF1 and illuminate physiological roles of a CPK-GEF-ROP module in ABA signaling and plant development. We further discuss that CPK-dependent RopGEF degradation during abiotic stress could provide a mechanism for down-regulation of RopGEF-dependent growth responses. © 2018 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163718
Appears in Collections:气候变化与战略

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作者单位: Li, Z., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States; Takahashi, Y., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States; Scavo, A., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States; Brandt, B., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States, Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, 1211, Switzerland; Nguyen, D., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States; Rieu, P., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States; Schroeder, J.I., Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, San Diego, CA 92093, United States

Recommended Citation:
Li Z.,Takahashi Y.,Scavo A.,et al. Abscisic acid-induced degradation of Arabidopsis guanine nucleotide exchange factor requires calcium-dependent protein kinases[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(19)
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