globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1719493115
论文题名:
Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki
作者: Mazin P.V.; Shagimardanova E.; Kozlova O.; Cherkasov A.; Sutormin R.; Stepanova V.V.; Stupnikov A.; Logacheva M.; Penin A.; Sogame Y.; Cornette R.; Tokumoto S.; Miyata Y.; Kikawada T.; Gelfand M.S.; Gusev O.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:10
起始页码: E2477
结束页码: E2486
语种: 英语
英文关键词: Anhydrobiosis ; Desiccation tolerance ; Heat shock ; Polypedilum vanderplanki ; RNA-seq
Scopus关键词: cell protein ; heat shock transcription factor ; late embryogenesis abundant protein ; thioredoxin ; transcriptome ; trehalose ; unclassified drug ; heat shock transcription factor ; insect protein ; anhydrobiosis ; Article ; carbohydrate metabolism ; cell line ; Chironomidae ; desiccation ; DNA structure ; Drosophila melanogaster ; gene activation ; gene function ; gene induction ; gene knockdown ; insect development ; nonhuman ; Polypedilum nubifer ; Polypedilum vanderplanki ; priority journal ; promoter region ; Pv11 cell line ; species comparison ; survival rate ; animal ; Chironomidae ; dehydration ; evolution ; female ; genetics ; heat shock response ; male ; metabolism ; physiological stress ; physiology ; Animals ; Biological Evolution ; Chironomidae ; Dehydration ; Female ; Heat Shock Transcription Factors ; Heat-Shock Response ; Insect Proteins ; Male ; Stress, Physiological
英文摘要: Polypedilum vanderplanki is a striking and unique example of an insect that can survive almost complete desiccation. Its genome and a set of dehydration–rehydration transcriptomes, together with the genome of Polypedilum nubifer (a congeneric desiccation-sensitive midge), were recently released. Here, using published and newly generated datasets reflecting detailed transcriptome changes during anhydrobiosis, as well as a developmental series, we show that the TCTAGAA DNA motif, which closely resembles the binding motif of the Drosophila melanogaster heat shock transcription activator (Hsf), is significantly enriched in the promoter regions of desiccation-induced genes in P. vanderplanki, such as genes encoding late embryogenesis abundant (LEA) proteins, thioredoxins, or trehalose metabolism-related genes, but not in P. nubifer. Unlike P. nubifer, P. vanderplanki has double TCTAGAA sites upstream of the Hsf gene itself, which is probably responsible for the stronger activation of Hsf in P. vanderplanki during desiccation compared with P. nubifer. To confirm the role of Hsf in desiccation-induced gene activation, we used the Pv11 cell line, derived from P. vanderplanki embryo. After preincubation with trehalose, Pv11 cells can enter anhydrobiosis and survive desiccation. We showed that Hsf knockdown suppresses trehalose-induced activation of multiple predicted Hsf targets (including P. vanderplanki-specific LEA protein genes) and reduces the desiccation survival rate of Pv11 cells fivefold. Thus, cooption of the heat shock regulatory system has been an important evolutionary mechanism for adaptation to desiccation in P. vanderplanki. © 2018 National Academy of Sciences. All Rights Reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163737
Appears in Collections:气候变化与战略

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作者单位: Mazin, P.V., Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, 143028, Russian Federation, Institute for Information Transmission Problems (Kharkevich Institute) RAS, Moscow, 127051, Russian Federation, Faculty of Computer Science, Higher School of Economics, Moscow, 119991, Russian Federation; Shagimardanova, E., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation; Kozlova, O., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation; Cherkasov, A., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation; Sutormin, R., Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94710, United States; Stepanova, V.V., Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, 143028, Russian Federation, Institute for Information Transmission Problems (Kharkevich Institute) RAS, Moscow, 127051, Russian Federation; Stupnikov, A., Department of Oncology, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, United States; Logacheva, M., Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, 143028, Russian Federation, Institute for Information Transmission Problems (Kharkevich Institute) RAS, Moscow, 127051, Russian Federation, A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russian Federation; Penin, A., Institute for Information Transmission Problems (Kharkevich Institute) RAS, Moscow, 127051, Russian Federation, A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russian Federation, Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russian Federation; Sogame, Y., Anhydrobiosis Research Group, Molecular Biomimetics Research Unit, Institute of Agrobiological Sciences, National Institute of Agriculture and Food Research Organization, Tsukuba, 305-0851, Japan, Japan Society for the Promotion of Science, Tokyo, 102-0083, Japan, Department of Applied Chemistry and Biochemistry, National Institute of Technology, Fukushima College, Iwaki, 970-8034, Japan; Cornette, R., Anhydrobiosis Research Group, Molecular Biomimetics Research Unit, Institute of Agrobiological Sciences, National Institute of Agriculture and Food Research Organization, Tsukuba, 305-0851, Japan; Tokumoto, S., Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan; Miyata, Y., Anhydrobiosis Research Group, Molecular Biomimetics Research Unit, Institute of Agrobiological Sciences, National Institute of Agriculture and Food Research Organization, Tsukuba, 305-0851, Japan, Center for Biological Resources and Informatics, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan; Kikawada, T., Anhydrobiosis Research Group, Molecular Biomimetics Research Unit, Institute of Agrobiological Sciences, National Institute of Agriculture and Food Research Organization, Tsukuba, 305-0851, Japan, Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan; Gelfand, M.S., Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, 143028, Russian Federation, Institute for Information Transmission Problems (Kharkevich Institute) RAS, Moscow, 127051, Russian Federation, Faculty of Computer Science, Higher School of Economics, Moscow, 119991, Russian Federation, Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119991, Russian Federation; Gusev, O., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation, RIKEN Innovation Center, RIKEN, Yokohama, 650-0047, Japan, Center for Life Science Technologies, RIKEN, Yokohama, 650-0047, Japan

Recommended Citation:
Mazin P.V.,Shagimardanova E.,Kozlova O.,et al. Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(10)
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