auxin
; cytokinin
; elongation factor
; elongation factor RSL4
; ethylene
; messenger RNA
; phosphorus
; plant protein
; regulator protein
; root hair defective 6 like 1 protein
; root hair defective 6 protein
; transcription factor
; transcription factor EIN3
; transcription factor EIN3 like 1 protein
; transcriptome
; unclassified drug
; Arabidopsis protein
; basic helix loop helix transcription factor
; DNA binding protein
; EIL3 protein, Arabidopsis
; EIN3 protein, Arabidopsis
; ethylene
; ethylene derivative
; nuclear protein
; RHD6 protein, Arabidopsis
; RSL1 protein, Arabidopsis
; transcription factor
; Arabidopsis
; Article
; gel mobility shift assay
; gene expression
; nonhuman
; plant organogenesis
; plant root
; priority journal
; protein protein interaction
; root growth
; root length
; Arabidopsis
; genetics
; growth, development and aging
; metabolism
; plant root
; Arabidopsis
; Arabidopsis Proteins
; Basic Helix-Loop-Helix Transcription Factors
; DNA-Binding Proteins
; Ethylenes
; Nuclear Proteins
; Plant Roots
; Transcription Factors
英文摘要:
Root hairs are an extensive structure of root epidermal cells and are critical for nutrient acquisition, soil anchorage, and environmental interactions in sessile plants. The phytohormone ethylene (ET) promotes root hair growth and also mediates the effects of different signals that stimulate hair cell development. However, the molecular basis of ET-induced root hair growth remains poorly understood. Here, we show that ET-activated transcription factor ETHYLENE-INSENSITIVE 3 (EIN3) physically interacts with ROOT HAIR DEFECTIVE 6 (RHD6), a well-documented positive regulator of hair cells, and that the two factors directly coactivate the hair length-determining gene RHD6-LIKE 4 (RSL4) to promote root hair elongation. Transcriptome analysis further revealed the parallel roles of the regulator pairs EIN3/EIL1 (EIN3-LIKE 1) and RHD6/ RSL1 (RHD6-LIKE 1). EIN3/EIL1 and RHD6/RSL1 coordinately enhance root hair initiation by selectively regulating a subset of core root hair genes. Thus, our work reveals a key transcriptional complex consisting of EIN3/EIL1 and RHD6/RSL1 in the control of root hair initiation and elongation, and provides a molecular framework for the integration of environmental signals and intrinsic regulators in modulating plant organ development.
Feng, Y., Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China, State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China; Xu, P., State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China; Li, B., Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China; Li, P., State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China; Wen, X., Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China; An, F., State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China; Gong, Y., State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China; Xin, Y., State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China; Zhu, Z., State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China; Wang, Y., Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China; Guo, H., Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China, Peking-Tsinghua Center of Life Sciences, Beijing, 100871, China
Recommended Citation:
Feng Y.,Xu P.,Li B.,et al. Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(52)