DOI: 10.1073/pnas.1703731114
论文题名: Escherichia coli responds to environmental changes using enolasic degradosomes and stabilized DicF sRNA to alter cellular morphology
作者: Murashko O.N. ; Lin-Chao S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期: 38 起始页码: E8025
结束页码: E8034
语种: 英语
英文关键词: Anaerobic conditions
; Cell shape
; Protein subcellular distribution
; RNA decay
; RNase E
Scopus关键词: chaperone
; enolase
; FtsZ protein
; multienzyme complex
; protein Hfq
; ribonuclease E
; small RNA DicF
; transfer RNA
; unclassified drug
; bacterial protein
; cytoskeleton protein
; enolase
; FtsZ protein, Bacteria
; ribonuclease
; ribonuclease E
; anaerobic growth
; Article
; bacterial cell
; bacterial strain
; cell division
; cell membrane
; cell structure
; cellular distribution
; comparative study
; controlled study
; environmental change
; enzyme activity
; enzyme degradation
; Escherichia coli
; nonhuman
; phase transition
; priority journal
; protein expression
; protein protein interaction
; regulatory mechanism
; RNA cleavage
; RNA degradation
; RNA stability
; Escherichia coli
; genetics
; metabolism
; physiology
; Anaerobiosis
; Bacterial Proteins
; Cytoskeletal Proteins
; Endoribonucleases
; Escherichia coli
; Phosphopyruvate Hydratase
英文摘要: Escherichia coli RNase E is an essential enzyme that forms multicomponent ribonucleolytic complexes known as “RNA degradosomes.” These complexes consist of four major components: RNase E, PNPase, RhlB RNA helicase, and enolase. However, the role of enolase in the RNase E/degradosome is not understood. Here, we report that presence of enolase in the RNase E/degradosome under anaerobic conditions regulates cell morphology, resulting in E. coli MG1655 cell filamentation. Under anaerobic conditions, enolase bound to the RNase E/degradosome stabilizes the small RNA (sRNA) DicF, i.e., the inhibitor of the cell division gene ftsZ, through chaperon protein Hfq-dependent regulation. RNase E/enolase distribution changes from membrane-associated patterns under aerobic to diffuse patterns under anaerobic conditions. When the enolase-RNase E/degradosome interaction is disrupted, the anaerobically induced characteristics disappear. We provide a mechanism by which E. coli uses enolase-bound degradosomes to switch from rod-shaped to filamentous form in response to anaerobiosis by regulating RNase E subcellular distribution, RNase E enzymatic activity, and the stability of the sRNA DicF required for the filamentous transition. In contrast to E. coli nonpathogenic strains, pathogenic E. coli strains predominantly have multiple copies of sRNA DicF in their genomes, with cell filamentation previously being linked to bacterial pathogenesis. Our data suggest a mechanism for bacterial cell filamentation during infection under anaerobic conditions. © 2017, National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163796
Appears in Collections: 气候变化与战略
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作者单位: Murashko, O.N., Institute of Molecular Biology, Academia Sinica, Taipei, 11529, Taiwan; Lin-Chao, S., Institute of Molecular Biology, Academia Sinica, Taipei, 11529, Taiwan
Recommended Citation:
Murashko O.N.,Lin-Chao S.. Escherichia coli responds to environmental changes using enolasic degradosomes and stabilized DicF sRNA to alter cellular morphology[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(38)