globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1703834114
论文题名:
Virus found in a boreal lake links ssDNA and dsDNA viruses
作者: Laanto E.; Mäntynen S.; De Colibus L.; Marjakangas J.; Gillum A.; Stuart D.I.; Ravantti J.J.; Huiskonen J.T.; Sundberg L.-R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期:31
起始页码: 8378
结束页码: 8383
语种: 英语
英文关键词: Cryo-electron microscopy ; Flavobacterium ; Genome ; Lipids ; Structure
Scopus关键词: capsid protein ; double stranded DNA ; fresh water ; RNA 16S ; single stranded DNA ; sucrose ; virus DNA ; capsid protein ; single stranded DNA ; Article ; controlled study ; double-stranded DNA virus ; electron microscopy ; lake ; microbial community ; nonhuman ; priority journal ; replicon ; RNA sequence ; single-stranded DNA virus ; sucrose gradient ; tandem mass spectrometry ; virion ; virus genome ; virus isolation ; virus purification ; classification ; DNA virus ; Enterobacteria phage PRD1 ; Flavobacterium ; genetics ; isolation and purification ; lake ; metabolism ; protein conformation ; virology ; virus capsid ; Bacteriophage PRD1 ; Capsid ; Capsid Proteins ; DNA Viruses ; DNA, Single-Stranded ; Flavobacterium ; Genome, Viral ; Lakes ; Protein Conformation
英文摘要: Viruses have impacted the biosphere in numerous ways since the dawn of life. However, the evolution, genetic, structural, and taxonomic diversity of viruses remain poorly understood, in part because sparse sampling of the virosphere has concentrated mostly on exploring the abundance and diversity of dsDNA viruses. Furthermore, viral genomes are highly diverse, and using only the current sequence-based methods for classifying viruses and studying their phylogeny is complicated. Here we describe a virus, FLiP (Flavobacterium-infecting, lipid-containing phage), with a circular ssDNA genome and an internal lipid membrane enclosed in the icosahedral capsid. The 9,174-nt-long genome showed limited sequence similarity to other known viruses. The genetic data imply that this virus might use replication mechanisms similar to those found in other ssDNA replicons. However, the structure of the viral major capsid protein, elucidated at near-atomic resolution using cryo-electron microscopy, is strikingly similar to that observed in dsDNA viruses of the PRD1–adenovirus lineage, characterized by a major capsid protein bearing two β-barrels. The strong similarity between FLiP and another member of the structural lineage, bacteriophage PM2, extends to the capsid organization (pseudo T = 21 dextro) despite the difference in the genetic material packaged and the lack of significant sequence similarity. © 2017, National Academy of Sciences. All rights reserved.
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被引频次[WOS]:38   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163809
Appears in Collections:气候变化与战略

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作者单位: Laanto, E., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland; Mäntynen, S., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland; De Colibus, L., Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom; Marjakangas, J., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland; Gillum, A., Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom; Stuart, D.I., Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom, Diamond Light Source, Didcot, OX11 0DE, United Kingdom; Ravantti, J.J., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland, Department of Biosciences, University of Helsinki, Helsinki, 00014, Finland; Huiskonen, J.T., Division of Structural Biology, Welcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom, Department of Biosciences, University of Helsinki, Helsinki, 00014, Finland; Sundberg, L.-R., Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, 40014, Finland, Nanoscience Center, University of Jyväskylä, Jyväskylä, 40014, Finland

Recommended Citation:
Laanto E.,Mäntynen S.,De Colibus L.,et al. Virus found in a boreal lake links ssDNA and dsDNA viruses[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(31)
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